| Herba houttuyniae extract induces apoptotic death of human promyelocytic leukemia cells via caspase activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release. | |
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MedLine Citation:
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PMID: 12754412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Herba houttuyniae has been used as a constituent of herval medicine prescriptions for the treatment of inflammation, cancer, and other diseases. In the present study, we investigated the cellular effects of herba houttuyniae extract (HHE) and the signal pathways of HHE-induced apoptosis in HL-60 human promyelocytic leukemia cell line. HHE treatment caused apoptosis of cells as evidenced by discontinuous fragmentation of DNA, the loss of mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of procaspase-9 and caspase-3, and proteolytic cleavage of poly(ADP-ribose) polymerase. Pretreatment of Ac-DEVD-CHO, caspase-3 specific inhibitor, or cyclosporin A, a mitochondrial permeability transition inhibitor, completely abolished HHE-induced DNA fragmentation. Together, these results suggest that HHE possibly causes mitochondrial damage leading to cytochrome c release into cytosol and activation of caspases resulting in PARP cleavage and execution of apoptotic cell death in HL-60 cells. |
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Authors:
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Kang-Beom Kwon; Eun-Kyung Kim; Byung-Cheul Shin; Eun-A Seo; Jeong-Yeh Yang; Do-Gon Ryu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental & molecular medicine Volume: 35 ISSN: 1226-3613 ISO Abbreviation: Exp. Mol. Med. Publication Date: 2003 Apr |
Date Detail:
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Created Date: 2003-05-19 Completed Date: 2004-01-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9607880 Medline TA: Exp Mol Med Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 91-7 Citation Subset: IM |
Affiliation:
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Department of Physiology, Chonbuk National University, Chonju 561-756, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Caspase 3 Caspases / metabolism* Cytochrome c Group / metabolism* Enzyme Activation / drug effects HL-60 Cells Humans Medicine, East Asian Traditional Membrane Potentials / drug effects Mitochondria / drug effects* Plant Extracts / pharmacology* Plants, Medicinal / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Cytochrome c Group; 0/Plant Extracts; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
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