Document Detail


Herba Cistanches stimulates cellular glutathione redoxcycling by reactive oxygen species generated from mitochondrial respiration in H9c2 cardiomyocytes.
MedLine Citation:
PMID:  23035909     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Context: Earlier findings demonstrated that pretreatment of Herba Cistanches [the dried whole plant of Cistanche deserticola Y.C. Ma (Orobanchaceae)], a "Yang-invigorating" Chinese tonic herb, stimulated the ATP-generation capacity (ATP-GC) in mitochondria isolated from rat heart ex vivo. The enhancement of mitochondrial ATP-GC by Herba Cistanches was associated with induction of glutathione antioxidant status and protection against ischemia/reperfusion (I/R) injury in rat hearts. Objectives: This study investigated the relationship between enhancements in mitochondrial ATP-GC and glutathione antioxidant status in H9c2 cardiomyocytes using a semipurified fraction of Herba Cistanches (HCF1). Materials and methods: HCF1 (10-300 ng/mL) was tested for its effects on mitochondrial ATP generation, glutathione antioxidant status and protection against oxidant injury in H9c2 cardiomyocytes and rat hearts. Results and discussion: HCF1 at 30 ng/mL increased mitochondrial ATP-GC and ADP-stimulated state 3 respiration (by 50 and 100%, respectively) in H9c2 cardiomyocytes. The stimulation of mitochondrial respiration was associated with the induction of mitochondrial uncoupling (27%) and enhancement of cellular glutathione redox cycling as well as protection against hypoxia/reoxygenation (hypox/reoxy)-induced apoptosis (by 60%). While HCF1 treatment increased reactive oxygen species generation from mitochondrial respiration in H9c2 cardiomyocytes, pretreatment with antioxidants (DMTU) abrogated the HCF1-induced cellular responses and the associated cytoprotective effect. HCF1 pretreatment (1.14 and 3.41 mg/kg × 14) also protected against myocardial I/R injury in rats (by 13 and 32%), presumably mediated by the induction of glutathione antioxidant response. Conclusion: The long-term intake of HCF1 may offer a prospect for the prevention of ischemic heart disease.
Authors:
Hoi Shan Wong; Kam Ming Ko
Related Documents :
9195299 - Interaction of orally administered 5-[3-[((2s)-1,4-benzodioxan-2-ylmethyl)amino]propoxy...
11769569 - Selective 5-ht1a receptor agonist, 8-oh-dpat, locally administered into the dorsal raph...
1832809 - Functional topography of brain serotonergic pathways in the rat.
2025379 - A quantitative autoradiographic study of serotonin1a receptor regulation. effect of 5,7...
19891989 - Behavioral, thermal and neurochemical effects of acute and chronic 3,4-methylenedioxyme...
19945469 - Cognitive impulsivity in animal models: role of response time and reinforcing rate in d...
2596249 - Changes in the connective tissue composition of the submucosal layer of colonic anastom...
20406669 - Chronic high corticosterone reduces neurogenesis in the dentate gyrus of adult male and...
9772669 - Effect of anisodine on acute forebrain ischemia-reperfusion damage in rats.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Pharmaceutical biology     Volume:  -     ISSN:  1744-5116     ISO Abbreviation:  Pharm Biol     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9812552     Medline TA:  Pharm Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Division of Life Science, Hong Kong University of Science & Technology Clear Water Bay , Hong Kong SAR , China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evaluation of recurrent hyphema after trabeculectomy with ultrabiomicroscopy 50-80 MHz: a case repor...
Next Document:  Regioselective Synthesis of Pyrazole Triflones Based on Triflyl Alkyne Cycloadditions.