| Hepoxilin B3 and its enzymatically formed derivative trioxilin B3 are incorporated into phospholipids in psoriatic lesions. | |
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MedLine Citation:
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PMID: 11851887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In previous studies we observed that normal human epidermis forms 12-oxo-eicosatetraenoic acid (12-oxo-ETE) and hepoxilin B3 (HxB3) as major eicosanoids, both being elevated in psoriasis. We also observed that normal epidermis, in a reaction probably catalyzed by 12-lipoxygenase, only synthesize one of the two possible 10-hydroxy epimers of HxB3. We have now extended these previous studies investigating further transformation of HxB3 into trioxilin B3 (TrXB3) and esterification of both into phospholipids. Phospholipids were extracted from normal epidermis and from psoriatic scales. A combination of high performance liquid chromatography and gas chromatography-mass spectrometry analysis demonstrated the occurrence of HxB3 and TrXB3 in the phospholipids of psoriatic lesions. Alkaline- and phospholipase-A2-mediated hydrolysis of the phospholipids yielded similar quantities of both HxB3 and TrXB3 indicating their preference for the sn-2 position of glycerophospholipids. The thin layer chromatography analysis of the phospholipid classes after incubation of epidermal cells with [14C]-labeled HxB3, TrXB3, 12-hydroxy-eicosatetraenoic acid (12-HETE), 12-oxo-ETE, or 15-HETE showed that 12-HETE was the most esterified (12-HETE >15-HETE > TrXB3 > 12-oxo-ETE > HxB3). HxB3 and TrXB3 were mainly esterified in phosphatidyl-choline and phosphatidyl-ethanolamine. HxB3 was also enzymatically converted into TrXB3 in vitro. HxB3 epoxide hydrolase-like activity was not observed when boiled tissue was incubated with [14C]-HxB3, this activity being located in the cytosol fraction (100,000 x g supernatant) of fresh tissue. These findings suggest that in vivo some part of HxB3 is transformed into TrXB3 and both compounds are partially incorporated into the phospholipids. |
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Authors:
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Rosa Antón; Mercedes Camacho; Luís Puig; Luís Vila |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of investigative dermatology Volume: 118 ISSN: 0022-202X ISO Abbreviation: J. Invest. Dermatol. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2002-02-19 Completed Date: 2002-07-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: United States |
Other Details:
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Languages: eng Pagination: 139-46 Citation Subset: IM |
Affiliation:
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Laboratory of Inflammation Mediators, Institute of Research of the Santa Creu i Sant Pau Hospital, Barcelona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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8,11,14-Eicosatrienoic Acid
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analogs & derivatives*,
metabolism* Cytosol / enzymology Epidermis / cytology, metabolism Epoxide Hydrolases / metabolism Esterification Humans Hydroxyeicosatetraenoic Acids / metabolism* Phosphatidylcholines / metabolism Phosphatidylethanolamines / metabolism Phospholipids / metabolism* Psoriasis / metabolism*, pathology* Reference Values Skin / metabolism, pathology |
| Chemical | |
Reg. No./Substance:
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0/Hydroxyeicosatetraenoic Acids; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids; 0/trioxilin B3; 71799-95-6/10-hydroxy-11,12-epoxyeicosa-5,8,14-trienoic acid; 7324-41-6/8,11,14-Eicosatrienoic Acid; EC 3.3.2.-/Epoxide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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