Document Detail

Hepatopancreas gluconeogenesis during hyposmotic stress in crabs Chasmagnathus granulata maintained on high-protein or carbohydrate-rich diets.
MedLine Citation:
PMID:  11126768     Owner:  NLM     Status:  MEDLINE    
The incorporation of [14C]-alanine or [14C]-lactate into glucose was measured in hepatopancreas fractions from Chasmagnathus granulata crabs adapted to a high protein or a carbohydrate-rich diet and submitted or not (control group) to hyposmotic stress. Gluconeogenic capacity and phosphoenolpyruvate carboxykinase (PEPCK) activity increased during acclimation to a dilute medium in C. granulata hepatopancreas. In intact animals, high hemolymph urea levels occurred for the high-protein regimen and for crabs fed both diets and submitted to hyposmotic stress. It could be that the amino acids released during hyposmotic stress are deaminated in the hepatopancreas, and that the carbon chains are used as substrate for gluconeogenesis. Hepatopancreas gluconeogenesis seems to be one of the pathways implicated in the metabolic adjustment of the amino acid pool during hyposmotic stress in C. granulata.
G T Oliveira; R S da Silva
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  127     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-20     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  375-81     Citation Subset:  IM    
Department of Physiology, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, ICBS, Porto Alegre, Brazil.
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MeSH Terms
Alanine / metabolism
Brachyura / metabolism*
Dietary Carbohydrates / metabolism*
Dietary Proteins / metabolism*
Digestive System / metabolism*
Glucose / metabolism*
L-Lactate Dehydrogenase / metabolism
Lactic Acid / metabolism
Osmotic Pressure
Phosphoenolpyruvate Carboxykinase (ATP) / metabolism
Reg. No./Substance:
0/Dietary Carbohydrates; 0/Dietary Proteins; 50-21-5/Lactic Acid; 50-99-7/Glucose; 56-41-7/Alanine; EC Dehydrogenase; EC Carboxykinase (ATP)

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