| Hepatoma cell migration through a mesothelial cell monolayer is inhibited by cyclic AMP-elevating agents via a Rho-dependent pathway. | |
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MedLine Citation:
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PMID: 11068034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1-Oleoyl lysophosphatidic acid (LPA) induces transmonolayer migration (in vitro invasion) of rat ascites hepatoma MM1 cells and their morphological changes leading to the migration. We have previously shown that an LPA analog, palmitoyl cyclic phosphatidic acid (Pal-cPA), suppresses transmonolayer migration of MM1 cells by rapidly increasing the intracellular cyclic AMP (cAMP) concentration. We report here that various cAMP-elevating agents, including dibutyryl cAMP, forskolin, cholera toxin and 3-isobutyl-1-methylxanthine, consistently inhibited LPA-induced transmonolayer migration of MM1 cells. Moreover, pull-down assays for GTP-bound, active RhoA demonstrated that the blockage by cAMP-elevating agents of morphological changes leading to the migration was probably mediated through inhibiting RhoA activation. |
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Authors:
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M Mukai; H Nakamura; M Tatsuta; T Iwasaki; A Togawa; F Imamura; H Akedo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 484 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-11-17 Completed Date: 2000-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 69-73 Citation Subset: IM |
Affiliation:
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Department of Tumor Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan. mmukai@mail.mc.pref.osaka.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bucladesine / pharmacology Carcinoma, Hepatocellular / pathology* Cell Movement / drug effects Cyclic AMP / metabolism* Drug Interactions Epithelium / pathology Liver Neoplasms / pathology* Lysophospholipids / pharmacology* Neoplasms, Mesothelial / pathology Rats Tumor Cells, Cultured rho GTP-Binding Proteins / metabolism* rhoA GTP-Binding Protein / antagonists & inhibitors, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lysophospholipids; 22002-87-5/lysophosphatidic acid; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; EC 3.6.5.2/rho GTP-Binding Proteins; EC 3.6.5.2/rhoA GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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