| Hepatoma-associated nonhistone chromosomal proteins. | |
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MedLine Citation:
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PMID: 6696912 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to compare nonhistone proteins in normal and neoplastic hepatocytes, we elicited antisera to Morris hepatoma 7777 dehistonized chromatin. By the enzyme-linked immunosorbent assay, the antisera demonstrated specificity for Morris hepatoma 7777 and little reactivity to normal rat liver chromatin. Morris hepatomas 7288c, 7800 and 5123tc shared some antigenic hepatoma nonhistone proteins. Neoplasia induced in rats fed 3'-methyl-4-dimethylaminoazobenzene changed the immunospecificity of the liver chromatin to a new type that was antigenically similar to Morris hepatoma 7777. Fetal rat liver chromatin and regenerating rat liver chromatin did not bind antibody. To further characterize the antigenic nonhistone proteins, we analyzed Morris hepatoma 7777 chromatin and normal rat liver chromatin by the immunoblot technique. Nonhistone proteins that demonstrated immunoreactivity were predominantly high molecular weight proteins. |
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Authors:
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A L Burkhardt; D P Huang; J F Chiu |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 781 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-04-11 Completed Date: 1984-04-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 165-72 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Transformation, Neoplastic / analysis Chromosomal Proteins, Non-Histone / immunology, metabolism* Liver / embryology Liver Neoplasms, Experimental / analysis*, chemically induced, metabolism Liver Regeneration Molecular Weight Rats |
| Grant Support | |
ID/Acronym/Agency:
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CA-25098/CA/NCI NIH HHS; PHS 5429-21-2/PH/PHPPO CDC HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromosomal Proteins, Non-Histone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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