Document Detail

Hepatocyte growth factor in gingival crevicular fluid and the distribution of hepatocyte growth factor-activator in gingival tissue from adult periodontitis.
MedLine Citation:
PMID:  12243969     Owner:  NLM     Status:  MEDLINE    
Hepatocyte growth factor (HGF), also known as scatter factor, is a broad-spectrum and multifunctional cytokine required for the development, growth and regeneration of various organs and tissues. The expression of HGF in human gingival fibroblasts is induced by inflammatory cytokines such as interleukin 1. Thus, although it is possible that content of HGF in gingival crevicular fluid (GCF) in periodontitis is increased, this has not so far been reported because the volume of GCF is too small to determine HGF by the available enzyme-linked immunosorbent assay (ELISA). A recently developed, highly sensitive ELISA for HGF, with a detection limit of 1 pg/ml sample, has now enabled HGF to be measured in GCF.The mean HGF content in GCF from sites with clinically healthy gingiva, defined by the absence of overt signs of gingival inflammation and a probing depth (PD) <3 mm, was 1.7 ng/ml, and that of periodontitis, defined by obvious alveolar bone loss detected by radiographic examination and a PD> or =3 mm, was 3.23 ng/ml. Although treating the periodontitis did not significantly decrease the HGF concentration despite significantly improved clinical scores such as PD and Gingival Index, the total amount of HGF in GCF did decrease significantly after treatment. HGF was expressed by gingival fibroblasts and inflammatory cells as determined by in situ hybridization. HGF-activator (HGFA), which converts inactive pro-HGF to active mature HGF, was detected in gingival epithelial cells by immunostaining. The expression of HGFA was also confirmed in gingival tissue by reverse transcription-polymerase chain reaction (RT-PCR). These findings indicate that HGF is synthesized and activated in gingiva that is clinically healthy or associated with periodontitis.
K Kakimoto; M Machigashira; T Ohnishi; T Kajihara; I Semba; T Setoguchi; M Tamura; Y Izumi; Y Daikuhara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  47     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-23     Completed Date:  2003-01-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  655-63     Citation Subset:  D; IM    
Department of Biochemistry, Kagoshima University Dental School, 35-1 Sakuragaoka-8, 890-8544, Kagoshima, Japan.
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MeSH Terms
Case-Control Studies
Enzyme-Linked Immunosorbent Assay / methods
Gene Expression
Gingival Crevicular Fluid / chemistry*
Hepatocyte Growth Factor / analysis*,  genetics
In Situ Hybridization
Middle Aged
Periodontitis / metabolism*
RNA / analysis
Reverse Transcriptase Polymerase Chain Reaction
Serine Endopeptidases / analysis*,  genetics
Reg. No./Substance:
63231-63-0/RNA; 67256-21-7/Hepatocyte Growth Factor; EC 3.4.21.-/HGF activator; EC 3.4.21.-/Serine Endopeptidases

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