| Hepatocyte growth factor attenuates eotaxin and PGD2-induced chemotaxis of human eosinophils. | |
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MedLine Citation:
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PMID: 17362253 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hepatocyte growth factor (HGF) is known to influence a number of cell types, and regulate various biologic activities including cell migration, proliferation, and survival. In a recent study, we found that, in vivo, HGF suppresses allergic airway inflammation, i.e. the infiltration of inflammatory cells including eosinophils into the airway, and further, that HGF reduces Th2 cytokine levels; however, the directly physiologic role of HGF with eosinophils remains unclear. In this study, we investigate the potential of recombinant HGF to regulate the factor-induced chemotaxis of human eosinophils. METHODS: Eosinophils were isolated from subjects with mild eosinophilia by modified CD16-negative selection. After culture with or without recombinant HGF, esoinophil chemotaxis was measured by Boyden chamber and KK chamber. RESULTS: Treatment with HGF prevented eotaxin or prostaglandin D(2) (PGD(2))-induced chemotaxis of eosinophils. Moreover, we demonstrated that extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinases as well as the enhancement of Ca(2+) influx, which are indispensable for eosinophil chemotaxis, were attenuated by HGF treatment. CONCLUSION: Taken together, these data suggest that in allergic diseases, HGF not only mediates eosinophils through the inhibition of Th2 cytokines, but also regulates the function of eosinophils directly, provides further insight into the cellular and molecular pathogenesis of allergic reactions. |
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Authors:
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W Ito; T Chiba; A Kanehiro; H Kato; K Yamaguchi; S Ueki; H Kayaba; J Chihara |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Allergy Volume: 62 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-16 Completed Date: 2007-05-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: Denmark |
Other Details:
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Languages: eng Pagination: 415-22 Citation Subset: IM |
Affiliation:
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Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Akita, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Calcium
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metabolism Cells, Cultured Chemokine CCL11 Chemokines, CC Chemotaxis / drug effects Eosinophilia / immunology, metabolism Eosinophils / drug effects*, immunology, metabolism Hepatocyte Growth Factor / pharmacology* Humans Prostaglandin D2 Receptors, CCR3 Receptors, Chemokine / metabolism Receptors, Immunologic / metabolism Receptors, Prostaglandin / metabolism Recombinant Proteins / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/CCL11 protein, human; 0/CCR3 protein, human; 0/Chemokine CCL11; 0/Chemokines, CC; 0/Receptors, CCR3; 0/Receptors, Chemokine; 0/Receptors, Immunologic; 0/Receptors, Prostaglandin; 0/Recombinant Proteins; 0/prostaglandin D2 receptor; 41598-07-6/Prostaglandin D2; 67256-21-7/Hepatocyte Growth Factor; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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