Document Detail


Hepatocyte cryopreservation: is it time to change the strategy?
MedLine Citation:
PMID:  20039443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liver cell transplantation presents clinical benefit in patients with inborn errors of metabolism as an alternative, or at least as a bridge, to orthotopic liver transplantation. The success of such a therapeutic approach remains limited by the quality of the transplanted cells. Cryopreservation remains the best option for long-term storage of hepatocytes, providing a permanent and sufficient cell supply. However, isolated adult hepatocytes are poorly resistant to such a process, with a significant alteration both at the morphological and functional levels. Hence, the aim of the current review is to discuss the state of the art regarding widely-used hepatocyte cryopreservation protocols, as well as the assays performed to analyse the post-thawing cell quality both in vitro and in vivo. The majority of studies agree upon the poor quality and efficiency of cryopreserved/thawed hepatocytes as compared to freshly isolated hepatocytes. Intracellular ice formation or exposure to hyperosmotic solutions remains the main phenomenon of cryopreservation process, and its effects on cell quality and cell death induction will be discussed. The increased knowledge and understanding of the cryopreservation process will lead to research strategies to improve the viability and the quality of the cell suspensions after thawing. Such strategies, such as vitrification, will be discussed with respect to their potential to significantly improve the quality of cell suspensions dedicated to liver cell-based therapies.
Authors:
Xavier Stéphenne; Mustapha Najimi; Etienne M Sokal
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Publication Detail:
Type:  Editorial; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-29     Completed Date:  2010-03-01     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  1-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Antioxidants / pharmacology
Cell Death
Cell Separation
Cell Survival
Cells, Cultured
Cryopreservation / methods*,  standards
Cryoprotective Agents / pharmacology
Hepatocytes* / drug effects,  metabolism,  pathology,  transplantation
Humans
Ice
Mitochondria, Liver / metabolism
Osmotic Pressure
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Cryoprotective Agents; 0/Ice
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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