| Hepatocarcinogenesis in transgenic mice carrying albumin-promoted SV40 T antigen gene. | |
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MedLine Citation:
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PMID: 1684356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have developed transgenic mice that inherit albumin promoter-regulated simian virus 40 (SV40) large T antigen gene, expressed specifically in hepatocytes. These mice all develop multifocal hepatocellular carcinomas at around 5 months and die of liver insufficiency by 7 months. Sequential morphological observation of hepatocarcinogenesis revealed 5 distinct stages: (I) newborn to 2 weeks of age, neither recognizable histological changes nor cellular replication in spite of T antigen expression; (II) between 3 and 7 weeks, diffuse cytomegalic change of hepatocytes with numerous abnormal mitoses, usually resulting in cell death; (III) from 7 weeks onwards, quasi-regenerative small hepatocyte foci with a decreased tendency for cytomegaly in spite of T antigen expression, rapidly replacing the hepatic tissue; (IV) 11 weeks of age and thereafter, neoplastic foci and nodules with enzymatic alteration; (V) 20 weeks of age and thereafter, gross hepatocellular carcinomas with occasional pulmonary metastases. Considerable variation existed both in morphological and enzymatic features and T antigen expression among neoplastic lesions, including carcinomas. Thus, these transgenic mice clearly show a multistep process in hepatocarcinogenesis with remarkable synchrony and provide a promising model for analyzing the essential events of carcinogenesis at different stages. |
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Authors:
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O Hino; T Kitagawa; K Nomura; K Ohtake; L Cui; Y Furuta; S Aizawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Japanese journal of cancer research : Gann Volume: 82 ISSN: 0910-5050 ISO Abbreviation: Jpn. J. Cancer Res. Publication Date: 1991 Nov |
Date Detail:
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Created Date: 1992-01-24 Completed Date: 1992-01-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8509412 Medline TA: Jpn J Cancer Res Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1226-33 Citation Subset: IM |
Affiliation:
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Department of Pathology, Cancer Institute, Tokyo. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Polyomavirus Transforming / genetics* Blotting, Northern Crosses, Genetic Female Glucose-6-Phosphatase / analysis Liver / cytology, microbiology, pathology* Liver Neoplasms, Experimental / genetics*, microbiology, pathology Male Mice Mice, Inbred Strains Mice, Transgenic Mitotic Index Plasmids Promoter Regions, Genetic* Restriction Mapping Serum Albumin / genetics* Simian virus 40 / genetics* Tumor Markers, Biological / analysis gamma-Glutamyltransferase / analysis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/Serum Albumin; 0/Tumor Markers, Biological; EC 2.3.2.2/gamma-Glutamyltransferase; EC 3.1.3.9/Glucose-6-Phosphatase |
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