Document Detail


Hepatobiliary handling of iodine-125-Tyr3-octreotide and indium-111-DTPA-D-Phe1-octreotide by isolated perfused rat liver.
MedLine Citation:
PMID:  8229254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Radiolabeled bioactive peptides may show receptor-mediated binding to tumors, making them suitable for scintigraphic imaging. The liver is an important organ for peptide clearance. To gain insight into the uptake and intracellular processing of somatostatin analogs, we compared the hepatobiliary handling of 125I-Tyr3-octreotide and 111In-DTPA-D-Phe1-octreotide, which are successfully used to image somatostatin receptor-positive tumors in vivo in isolated recirculating perfused rat livers. Sixty minutes following administration of the radiolabeled peptides, perfusion medium and biliary radioactivity were analyzed. Radioiodinated Tyr3-octreotide was rapidly cleared by the liver and 60% of the dose was excreted intact into the bile after 60 min. In contrast, 111In-DTPA-D-Phe1-octreotide was not cleared by the liver; medium radioactivity levels remained about constant and only 2% of the dose was found in the bile. These results are in agreement with in vivo findings in rats and humans. We concluded that isolated rat liver perfusion is a good system to rapidly gain insight into the hepatic handling of radiopharmaceuticals.
Authors:
M de Jong; W H Bakker; W A Breeman; M E van der Pluijm; P P Kooij; T J Visser; R Docter; E P Krenning
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of nuclear medicine : official publication, Society of Nuclear Medicine     Volume:  34     ISSN:  0161-5505     ISO Abbreviation:  J. Nucl. Med.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-11-29     Completed Date:  1993-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217410     Medline TA:  J Nucl Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2025-30     Citation Subset:  IM    
Affiliation:
Department of Nuclear Medicine, University Hospital Dijkzigt, Rotterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile / metabolism*
Liver / metabolism*
Male
Octreotide / analogs & derivatives*,  pharmacokinetics
Pentetic Acid / analogs & derivatives*,  pharmacokinetics
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
142694-57-3/SDZ 215-811; 67-43-6/Pentetic Acid; 83150-76-9/Octreotide
Comments/Corrections
Comment In:
J Nucl Med. 1993 Nov;34(11):2030-1   [PMID:  7901352 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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