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Hepatitis E virus seroprevalence among adults, Germany.
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PMID:  23018055     Owner:  NLM     Status:  MEDLINE    
We assessed hepatitis E virus (HEV) antibody seroprevalence in a sample of the adult population in Germany. Overall HEV IgG prevalence was 16.8% (95% CI 15.6%-17.9%) and increased with age, leveling off at >60 years of age. HEV is endemic in Germany, and the lifetime risk for exposure is high.
Mirko S Faber; Jürgen J Wenzel; Wolfgang Jilg; Michael Thamm; Michael Höhle; Klaus Stark
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Emerging infectious diseases     Volume:  18     ISSN:  1080-6059     ISO Abbreviation:  Emerging Infect. Dis.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-02-19     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9508155     Medline TA:  Emerg Infect Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1654-7     Citation Subset:  IM    
Robert Koch Institute, Berlin, Germany.
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MeSH Terms
Germany / epidemiology
Hepatitis Antibodies / blood*
Hepatitis E / epidemiology*,  virology
Hepatitis E virus / immunology*
Immunoglobulin G / blood
Middle Aged
Seroepidemiologic Studies
Young Adult
Reg. No./Substance:
0/Hepatitis Antibodies; 0/Immunoglobulin G

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Journal Information
Journal ID (nlm-ta): Emerg Infect Dis
Journal ID (iso-abbrev): Emerging Infect. Dis
Journal ID (publisher-id): EID
ISSN: 1080-6040
ISSN: 1080-6059
Publisher: Centers for Disease Control and Prevention
Article Information
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Print publication date: Month: 10 Year: 2012
Volume: 18 Issue: 10
First Page: 1654 Last Page: 1657
PubMed Id: 23018055
ID: 3471611
Publisher Id: 11-1756
DOI: 10.3201/eid1810.111756

Hepatitis E Virus Seroprevalence among Adults, Germany Alternate Title:Hepatitis E Virus Seroprevalence, Germany
Mirko S. Faber1
Jürgen J. Wenzel1
Wolfgang Jilg
Michael Thamm
Michael Höhle
Klaus Stark
Robert Koch Institute, Berlin, Germany (M.S. Faber, M. Thamm, M. Höhle, K. Stark);
and University of Regensburg, Regensburg, Germany (J.J. Wenzel, W. Jilg)
Correspondence: Address for correspondence: Mirko S. Faber, Department for Infectious Disease Epidemiology, Robert Koch Institute, DGZ-Ring 1, 13086 Berlin, Germany; email:

In industrialized countries, hepatitis E virus (HEV) has long been regarded as a rare imported infection. However, sporadic cases without travel to disease-endemic areas and caused by genotype 3 are being increasingly reported (1,2). Epidemiologic and molecular studies have implicated undercooked pork and wild boar products as a source of HEV infection (35). An unexpectedly high prevalence of HEV-specific antibodies, e.g., among blood donors, has been shown by several studies in Europe and the United States (611).

In Germany, the number of notified hepatitis E cases rose from <50 annually in 2001–2003 to 238 in 2011 (incidence 0.3/100,000 population); the proportion of autochthonous cases increased from 30%–40% to 78%. We conducted a study to determine HEV seroprevalence in Germany’s adult population and associations with sociodemographic characteristics by using an assay highly sensitive for HEV genotype 3.

The Study

We assessed HEV seroprevalence in a large subsample (n = 4,422) of the 2008–2011 German Health Examination Survey for Adults (Deutscher Erwachsenen Gesundheitssurvey;, a 2-stage national probability sample that assessed the health status in the general population. The sampling frame comprised persons 18–79 years of age whose principal residence was in Germany and who were fluent in German. Overall response was 48.4% (7,116 respondents). Our subsample reflects the total adult population with respect to age, sex, and geographic region, but persons with migration background are underrepresented (non-German citizenship 4.6% in the sample vs. 8.7% in the total adult population).

Serum samples were screened for HEV IgG by using the recomLine HEV-IgG/IgM immunoassay (Mikrogen, Neuried, Germany). The assay is based on 7 recombinantly expressed antigens of genotypes 1 and 3 of open reading frames 2 and 3. According to the manufacturer’s and our data (J.J. Wenzel et al., unpub. data), the test is 97%–100% sensitive for detecting acute or previous HEV infections. Test strips were scanned with the semiautomatic recomScan software (Mikrogen). The intensity of 3 quality assurance and other bands was determined by densitometrical detection algorithms. Each antigen band with an intensity greater or equal to the cutoff was assigned a point value. The final results were classified into 3 categories: no antibodies detectable (negative), test inconclusive (borderline), and antibodies detectable (positive). Persons whose test results were borderline (n = 70) were excluded from further analysis.

We poststratified the remaining survey population (n = 4,352) by age group and location of residence (16 states) to account for per protocol oversampling in eastern Germany and to restore the distribution of age groups to match the distribution in the total population. Weighted seroprevalence estimates were calculated by using survey-weighted logistic regression. Associations between demographic characteristics and seropositivity were analyzed by using adjusted Wald test p values. We also estimated mean annual incidence of HEV seroconversion from the seroprevalence data by using a catalytic model with age-constant force of infection, similar to that of Faramawi et al. (12). Detailed methods and underlying assumptions are described in the Technical Appendix.

The 4,352 persons who were included in the analysis were from 108 communities of all federal states in Germany (Table 1). Weighted prevalence of HEV IgG was 16.8% (95% CI 15.6%–17.9%); prevalence ranged from 6.1% (95% CI 4.5%–7.8%) in the 18–34-year age group to >20% in the >50-year groups, with a maximum of 26.4% (95% CI 21.6%–31.1%) in the 60–64-year group (Figure). In the univariable analysis (Table 2), only age group was significantly associated with seropositivity (p<0.01); results were not significant for sex (p = 0.97), residence (northern/middle/southern Germany, p = 0.29; west/east, p = 0.43), or population of municipality (4 categories; p = 0.10). In separate multivariable models, each including age group and 1 other variable, age remained the only significant variable. Mean annual incidence of HEV seroconversion estimated from the catalytic model was 3.9 (95% CI 3.6%–4.2%) per 1,000 population.


We found an overall HEV seroprevalence of 16.8% among adults in Germany; seroprevalence increased with age but was not dependent on sex or location of residence. Similarly high seroprevalence was found in blood donors in Denmark (20.6%), southwestern England (16%), and the United States (18%) (6,7,9). Estimates from Switzerland and the Netherlands, on the other hand, are considerably lower (10,11). Reasons for these differences could be effects of sample selection, different lifetime exposures (e.g., to foods that may serve as transmission vehicles), or use of different test systems with varying sensitivity. Mansuy et al. recently reported 53% prevalence of HEV antibodies in blood donors in southwestern France (13), a figure considerably higher than the 17% prevalence reported earlier for the same geographic region, when a different test system was used (8). The assay applied in our study was designed to also detect previous infections with HEV genotype 3; therefore, it is likely to be more sensitive than assays used in previous studies.

Our data show that HEV exposure is common among the general adult population in Germany, which is consistent with increasing evidence for pigs as a reservoir for foodborne transmission of HEV in industrialized countries. HEV seroprevalence is high in domestic pig herds in Germany and other countries; closely related HEV strains were found in pig livers on retail sale and in autochthonous cases of hepatitis E, and HEV seroprevalence was higher in persons with occupational exposure to pigs than in control groups (4,10,14,15).

Our data and other studies (1114) have shown no significant difference in seroprevalence between sexes, despite an assumed higher frequency of alimentary or occupational exposure and the higher incidence of clinical cases among men. This finding may indicate sex-specific differences in disease development or application of laboratory testing or that foods frequently consumed by both sexes play a substantial role as vehicles for transmission. These results also may highlight the lack of evidence for 1 main risk factor or food vehicle (14).

The strong association between age and HEV seroprevalence in our study most likely reflects cumulative lifetime exposure to the virus. However, HEV seroprevalence remains relatively stable from 18 to 34 years of age, which could indicate a birth cohort effect resulting from a decrease in the overall risk over the past few decades, as was reported for Denmark (6). The leveling off of the seroprevalence above age 60 years could be caused by a loss of antibodies in the elderly.

We found a striking difference between the estimated annual incidence of seroconversion and the relatively low incidence of notified disease in Germany. Besides underdiagnosis, a possible explanation is a high proportion of asymptomatic infections.

The survey response rate is similar to those achieved in other comprehensive national health examination surveys in Europe. In the statistical analysis, we corrected for differences in the distribution of age and place of residence between the sample and the general population. However, foreign-born persons are underrepresented in the survey because participation required fluency in German. A high proportion of foreign-born persons in Germany are Muslim, and their avoidance of raw pork products likely decreases their risk for HEV infection, which means we may have slightly overestimated the seroprevalence in the adult general population.

Autochthonous HEV infections are common in the industrialized world, and testing for HEV is indicated in patients with acute hepatitis, irrespective of history of travel to developing countries. Recommendations against eating undercooked pork products exist, but further studies are needed to specify implicated food products and to better target recommendations for safer food production, preparation, and consumption.

Technical Appendix

Details of mathematical methods used to compute the yearly incidence from the seroprevalence data in the manuscript.


Suggested citation for this article: Faber MS, Wenzel JJ, Jilg W, Thamm M, Höhle M, Stark K. Hepatitis E virus seroprevalence among adults, Germany. Emerg Infect Dis [Internet]. 2012 Oct [date cited].

1These authors contributed equally to this article.


We thank Monika Erl and Bianka Ehrlich for expert technical assistance and Heribert Stolzenberg and Panagiotis Kamtsiuris for critical review of the manuscript.

No external funding was received for this study. The study was approved by the ethical review board of the Charité University Hospital, Berlin.

Dr Faber is a medical epidemiologist at the Robert Koch Institute, Department for Infectious Disease Epidemiology (Gastrointestinal Infections, Zoonoses and Tropical Infections Unit).

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[Figure ID: F1]

Estimated prevalence of hepatitis E virus (HEV) IgG, by age group, Germany, 2008–2011. Error bars indicate 95% CIs.

[TableWrap ID: T1] Table 1  Comparison of demographic characteristics of persons in study of hepatitis E virus seroprevalence and general adult population, Germany, 2008–2011
Characteristic % Study population, n = 4,352 % Total population, n = 64,139,871*
Age group, y
18–19 3.2 2.9
20–29 12.3 15.5
30–39 11.5 15.6
40–49 18.4 21.7
50–59 19.5 17.9
60–69 19.7 14.3
70–79 15.4 12.2
Female sex 51.3 50.3
State of residence
Baden-Württemberg 11.4 13.0
Bavaria 10.7 15.2
Berlin 3.4 4.4
Brandenburg 6.2 3.2
Bremen 0.9 0.8
Hamburg 1.4 2.2
Hesse 6.4 7.4
Mecklenburg-Vorpommern 3.8 2.1
Lower Saxony 11.2 9.5
North Rhine-Westphalia 14.8 21.6
Rhineland-Palatinate 5.1 4.8
Saarland 2.2 1.3
Saxony 7.1 5.3
Saxony-Anhalt 6.2 3.0
Schleswig-Holstein 2.6 3.4
Thuringia 6.7 2.9

*Total no. adults 18–79 years of age as of December 31, 2009 (

[TableWrap ID: T2] Table 2  Univariable survey-weighted logistic regression estimates of seroprevalence of hepatitis E virus IgG, by age, sex, and location of residence, Germany, 2008–2011*
Characteristic Seroprevalence, % (95% CI)
Total 16.8 (15.6–17.9)
Age group, y
18–19 5.3 (2.5–11.1)
20–29 6.6 (4.8–9.2)
30–39 8.0 (5.8–10.8)
40–49 16.9 (14.3–19.9)
50–59 22.6 (19.7–25.7)
60–69 26.1 (23.0–29.4)
70–79 23.8 (20.4–27.5)
M 16.8 (15.2–18.5)
F 16.7 (15.2–18.5)
Place of residence (north–south)
Northern states 15.5 (13.5–17.6)
Middle states 16.6 (14.8–18.6)
Southern states 17.9 (15.8–20.1)
Place of residence (east-west)
Western states 16.6 (15.2–18.0)
Eastern states 17.6 (15.6–19.7)
Population of municipality
<5,000 17.9 (15.3–20.8)
5,000–<20,000 17.5 (15.2–20.1)
20,000–<100,000 14.4 (12.4–16.6)
>100,000 17.7 (15.7–20.0)

*n = 4,352.

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Keywords: Keywords: Hepatitis E virus, cross-sectional study, HEV, hepatitis E, seroprevalence, Germany, viruses, hepatitis, adults.

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