Document Detail

Hepatitis C virus infection of a Vero cell clone displaying efficient virus-cell binding.
MedLine Citation:
PMID:  9108623     Owner:  NLM     Status:  MEDLINE    
The susceptibility of Vero cells and derivative cell clones to hepatitis C virus (HCV) infection was assayed by qualitative and quantitative polymerase chain reaction (PCR)-based methods. Cell extracts from Vero cells inoculated with HCV were tested for the presence of both positive and negative strands of HCV RNA; in parallel, cell-free HCV genomes were assayed in culture supernatant fluids. Quantitation of genomic HCV RNA molecules in infected cells by competitive reverse transcription PCR (cRT-PCR) indicated that HCV replication was more efficient in a derivative clone (named clone 10) than in parental Vero cells or other clones under study. Analysis of HCV-binding to cell receptors, performed by cRT-PCR quantitation of viral particles adsorbed to the cell surface, demonstrated a 10-fold higher virus-binding level of clone 10 than that of parental Vero cells. The results shown here indicate that the Vero clone 10 may constitute an efficient model system for analysing early events in HCV infection as well as a source of virus for diagnostic and biotechnological applications.
M B Valli; G Carloni; A Manzin; F Nasorri; A Ponzetto; M Clementi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Research in virology     Volume:  148     ISSN:  0923-2516     ISO Abbreviation:  Res. Virol.     Publication Date:    1997 Mar-Apr
Date Detail:
Created Date:  1997-06-23     Completed Date:  1997-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907469     Medline TA:  Res Virol     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  181-6     Citation Subset:  IM    
Istituto di Medicina Sperimentale, Consiglio Nazionale delle Ricerche (C.N.R.), Roma, Italy.
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MeSH Terms
Cercopithecus aethiops
Clone Cells
Hepacivirus / physiology*
Membrane Fusion*
Polymerase Chain Reaction
Vero Cells
Virus Replication

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