Document Detail

Hepatitis C virus NS5A protein protects against TNF-alpha mediated apoptotic cell death.
MedLine Citation:
PMID:  10867196     Owner:  NLM     Status:  MEDLINE    
Hepatitis C virus (HCV) often causes a prolonged and persistent infection which may lead to hepatocellular carcinoma. We have previously reported that the nonstructural 5A (NS5A) protein of HCV promotes cell growth [Ghosh, A.K., Steele, R., Meyer, K., Ray, R., Ray, R.B., 1999. Hepatitis C virus NS5A protein modulates cell cycle regulatory genes and promotes cell growth. J. Gen. Virol. 80, 1179-1183]. In this study, we investigated the role of HCV NS5A (genotype 1a, strain H) in TNF-alpha induced apoptotic cell death. HepG2 cells expressing NS5A exhibited an inhibitory role in relation to TNF-alpha mediated apoptotic cell death. The NS5A protein blocked the activation of caspase-3 and inhibited proteolytic cleavage of the death substrate poly (ADP-ribose) polymerase in TNF-alpha induced cells. Together, these results suggest that HCV NS5A protein protects against TNF-alpha mediated apoptotic cell death.
A K Ghosh; M Majumder; R Steele; K Meyer; R Ray; R B Ray
Related Documents :
15199146 - Essential roles of receptor-interacting protein and traf2 in oxidative stress-induced c...
14959856 - Physiological oxidants induce apoptosis and cell cycle arrest in a multidrug-resistant ...
1388096 - Apoptosis, cell proliferation and c-ras expression during and after cyproterone acetate...
19446606 - A novel wd-40 repeat protein wdr26 suppresses h2o2-induced cell death in neural cells.
17909856 - Varicella-zoster virus infection induces the secretion of interleukin-8.
24937256 - Bioenergetics in chicken embryo fibroblast cells: evidence of lower proton leak in spon...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virus research     Volume:  67     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-08-09     Completed Date:  2000-08-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  173-8     Citation Subset:  IM    
Department of Pathology, Saint Louis University, St. Louis, MO 63104, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blotting, Western
Caspase 3
Caspases / analysis,  metabolism
Cell Death* / drug effects
Cell Line
Dose-Response Relationship, Drug
Hepacivirus / chemistry*
Microscopy, Confocal
Poly(ADP-ribose) Polymerases
Proteins / analysis,  metabolism
Recombinant Proteins / biosynthesis
Time Factors
Tumor Necrosis Factor-alpha / genetics,  pharmacology*
Viral Nonstructural Proteins / biosynthesis,  pharmacology,  physiology*
Grant Support
Reg. No./Substance:
0/NS-5 protein, hepatitis C virus; 0/Proteins; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 0/Viral Nonstructural Proteins; EC protein, human; EC Polymerases; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rearrangement generated in double genes, NSP1 and NSP3, of viable progenies from a human rotavirus s...
Next Document:  Inhibition of influenza C viruses by human MxA protein.