| Hepatitis B virus surface antigen interacts with acid alpha-glucosidase and alters glycogen metabolism. | |
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MedLine Citation:
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PMID: 20618459 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Aim: Hepatitis B virus (HBV) infection is highly correlated with hepatocellular carcinoma. Previous studies have reported that expression of hepatitis B virus pre-S2 mutant surface antigen is related to hepatoma development. An aberrant carbohydrate metabolism is a hallmark of malignant transformation. Methods: We performed yeast two-hybrid screening with HBV pre-S2-del large surface protein (pre-S2Delta) by using human liver cDNA library, and identified the acid alpha-glucosidase (acid alpha-glucosidase) as the novel cellular interacting protein of pre-S2Delta. The association of pre-S2Delta with the acid alpha-glucosidase was confirmed by confocal immunofluorescence and co-immunoprecipitation assay. Further, the acid alpha-glucosidase activity and glycogen content were analyzed in ML-1 cells expressing pre-S2Delta. Results: The interaction between HBV large surface protein and acid alpha-glucosidase was demonstrated with co-immunoprecipitation in vitro and in vivo, and the binding was mediated through c-terminal region 889-952 amino acid of acid alpha-glucosidase. On the other hand, HBV large surface protein interacted with acid alpha-glucosidase through N-terminal region 1-157 amino acid of HBV large surface protein. Expression of HBV large surface protein enhanced acid alpha-glucosidase activity and resulted in decrease of cellular glycogen. Conclusion: Our result demonstrates that HBV large surface protein interacts with acid alpha-glucosidase which plays an important role in glycogen balance. Together, these data suggest a novel pathway by which HBV large surface protein affects carbohydrate metabolism. |
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Authors:
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Jui-Hsiang Hung; Chiao-Wen Yan; Ih-Jen Su; Hui-Ching Wang; Huan-Yao Lei; Wan-Chi Lin; Wen-Tsan Chang; Wenya Huang; Te-Jung Lu; Ming-Derg Lai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Hepatology research : the official journal of the Japan Society of Hepatology Volume: 40 ISSN: 1386-6346 ISO Abbreviation: Hepatol. Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9711801 Medline TA: Hepatol Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 633-40 Citation Subset: - |
Affiliation:
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Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan, Taiwan. Email: a1211207@mail.ncku.edu.tw. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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