| Hepatitis B virus enhances transduction of human hepatocytes by SV40-based vectors. | |
| | |
MedLine Citation:
|
PMID: 15123369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND/AIMS: Chronic HBV infection, a world-wide epidemic, can lead to chronic hepatitis and eventually to cirrhosis and hepatocellular carcinoma. The liver poses obstacles for many available gene-transfer vectors. SV40-based vectors can transduce human hepatic and hematopoietic cells. We studied the effect of HBV on the transduction - efficiency of human hepatic cells by SV40 - based vectors. METHODS: A SV40-vector carrying the luciferase gene, and wild-type SV40, were used to assess transduction efficiency of human HBV-positive and HBV-negative hepatic cells. Transduction efficiency was measured as luciferase activity or by T-antigen staining. To evaluate whether differences in transduction efficiency are due to cell recognition and/or nuclear transport, MHC-I receptors were measured by FACS analysis and SV40-DNA was extracted from the nuclei of transduced cells and quantified. RESULTS: Two HBV-positive cell-lines, HepG2.2.2.15 and FLC4-A10II, were transduced significantly more efficiently than their parental HBV-negative cell-lines. Transient transfection of HuH-7 cells with the HBV genome also increased transduction efficiency. The level of MHC-I, the cellular receptor for SV40, was comparable in all the cell-lines studied. However, soon after infection with SV40, the nuclei of HepG2.2.2.15 contained >6-fold more SV40-DNA than HepG2. CONCLUSIONS: HBV increases transduction by SV40-vectors. This is due to enhanced vector entry and/or transport into the nucleus. SV40-vectors appear to have a potential for gene therapy for the treatment of HBV infections. |
| | |
Authors:
|
Uri Arad; Jonathan Axelrod; Orly Ben-nun-Shaul; Ariella Oppenheim; Eithan Galun |
Related Documents
:
|
19771229 - Potent subunit-specific effects on cell growth and drug sensitivity from optimised sirn... 8822269 - Processing of pro-opiomelanocortin in gh3 cells: inhibition by prohormone convertase 2 ... 3164719 - Characterization of cloned human leukocyte 5-lipoxygenase expressed in mammalian cells. 1847319 - Two strains of human keratinocytes transfected with hpv16 dna: comparison with the norm... 10085259 - Polarized targeting of epithelial cell proteins in thyrocytes and mdck cells. 11342269 - Construction of tumor-specific cells expressing a membrane-anchored single-chain fv of ... 3437819 - Mobile phospholipid signals in nmr spectra of cultured human adenocarcinoma cells. 22745829 - Mirna expression profiling in migrating glioblastoma cells: regulation of cell migratio... 19342029 - The variable expression of lectin-like oxidized low-density lipoprotein receptor (lox-1... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of hepatology Volume: 40 ISSN: 0168-8278 ISO Abbreviation: J. Hepatol. Publication Date: 2004 Mar |
Date Detail:
|
Created Date: 2004-05-04 Completed Date: 2004-11-10 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: England |
Other Details:
|
Languages: eng Pagination: 520-6 Citation Subset: IM |
Affiliation:
|
Department of Hematology and Goldyne Savad Gene Therapy Institute, The Hebrew University-Hadassah Medical School and Hadassah University Hospital, Jerusalem, Israel. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Cell Nucleus / metabolism DNA, Viral / metabolism Dimerization Gene Expression Genetic Vectors* Genome, Viral Hepatitis B virus / metabolism, physiology* Hepatocytes / physiology* Histocompatibility Antigens Class I / metabolism Humans Polyomavirus Infections / metabolism Promoter Regions, Genetic Receptors, Virus / metabolism Simian virus 40 / genetics* Transduction, Genetic* Transfection Tumor Virus Infections / metabolism Viral Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Viral; 0/Histocompatibility Antigens Class I; 0/Receptors, Virus; 0/Viral Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Non-sequencing molecular approaches to identify preS2-defective hepatitis B virus variants proved to...
Next Document: Hepatitis C virus Core Antigen as a predictor of non-response in genotype 1 chronic hepatitis C pati...