Document Detail


Hepatitis B virus X protein upregulates expression of calpain small subunit 1 via nuclear factor-kappaB/p65 in hepatoma cells.
MedLine Citation:
PMID:  20419804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hepatitis B virus (HBV) infection is closely correlated with the development of hepatocellular carcinoma (HCC), in which hepatitis B virus X protein (HBx) plays crucial roles. HBx is believed to be a multifunctional oncoprotein. It has been reported that the calpain small subunit 1 (Capn4) is upregulated in the HCC tissues and involved in the metastasis of HCC. Therefore, we suppose that HBx may promote hepatoma cell migration through Capn4. In the present study, we investigated the effect of HBx on regulating Capn4 expression in human HCC cells. Our data showed that HBx could increase promoter activity of Capn4 and upregulate the expression of Capn4 at the levels of mRNA and protein in human hepatoma HepG2 (or H7402) cells using luciferase reporter gene assay, real-time quantitative RT-PCR assay and Western blot analysis. While, the RNA interference targeting HBx mRNA was able to abolish the upregulation. Interestingly, we found that the inhibition of nuclear factor-kappaB (NF-kappaB) mediated by siRNA targeting NF-kappaB/p65 mRNA or PDTC (an inhibitor of NF-kappaB) could attenuate the upregulation of Capn4. While, HBx failed to increase the promoter activity of Capn4 in hepatoma cells when the putative NF-kappaB binding site of the Capn4 promoter was mutant, suggesting that NF-kappaB is involved in the activation of Capn4 mediated by HBx. In function, wound healing assay showed that HBx could significantly enhance the migration ability of HepG2 cells through upregulating Capn4. Thus, we conclude that HBx upregulate Capn4 through NF-kappaB/p65 to promote migration of hepatoma cells.
Authors:
Feng Zhang; Qi Wang; Lihong Ye; Yingming Feng; Xiaodong Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  82     ISSN:  1096-9071     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  920-8     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Affiliation:
Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China. fengym@fmmu.edu.cn
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Calpain / biosynthesis*
Cell Line, Tumor
Cell Movement
Genes, Reporter
Hepatitis B virus / pathogenicity*
Hepatocytes / virology*
Host-Pathogen Interactions*
Humans
Luciferases / biosynthesis,  genetics
Protein Biosynthesis
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Trans-Activators / metabolism*
Transcription Factor RelA / metabolism*
Up-Regulation
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Trans-Activators; 0/Transcription Factor RelA; 0/hepatitis B virus X protein; EC 1.13.12.-/Luciferases; EC 3.4.22.-/Calpain; EC 3.4.22.52/CAPNS1 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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