Document Detail

Hepatitis B virus RNA is measurable in serum and can be a new marker for monitoring lamivudine therapy.
MedLine Citation:
PMID:  16988768     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Changes in the serum hepatitis B virus (HBV) RNA level during lamivudine therapy were compared to those in the serum HBV DNA and HBV core-related antigen (HBVcrAg) levels in 24 patients with chronic hepatitis B. METHODS: For measurement of HBV RNA, total nucleic acid was extracted from serum samples and treated with RNase-free DNase I. After cDNA synthesis from extracted RNA, HBV RNA was measured by real-time detection polymerase chain reaction. RESULTS: The peak fraction of HBV RNA in serum samples was consistent with peak fractions of HBV DNA and HBV core protein in a sucrose gradient analysis, indicating that HBV RNA was incorporated into virus particles. All levels of HBV DNA, HBV RNA, and HBVcrAg decreased gradually during lamivudine therapy (P < 0.001 for all). The amount of decrease from the start of lamivudine therapy was significantly higher for HBV DNA than for HBV RNA or HBVcrAg during 6 months of lamivudine therapy (P < 0.001 for all). However, a similar difference was not seen between HBV RNA and HBVcrAg levels during that period. The HBV RNA level was significantly correlated (P < 0.001 for all) with levels of HBV DNA and HBVcrAg both at the beginning and 2 months after the start of lamivudine therapy. CONCLUSIONS: HBV RNA is detectable in serum in a form indicating incorporation into virus particles, and its serum level might serve as a new viral marker with a significance different from that of HBV DNA in lamivudine therapy.
Akinori Rokuhara; Akihiro Matsumoto; Eiji Tanaka; Takeji Umemura; Kaname Yoshizawa; Tatsuji Kimura; Noboru Maki; Kendo Kiyosawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of gastroenterology     Volume:  41     ISSN:  0944-1174     ISO Abbreviation:  J. Gastroenterol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-21     Completed Date:  2006-12-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430794     Medline TA:  J Gastroenterol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  785-90     Citation Subset:  IM    
Department of Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.
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MeSH Terms
Centrifugation, Density Gradient
DNA, Viral / blood
Hepatitis B Core Antigens / blood
Hepatitis B virus / isolation & purification*
Hepatitis B, Chronic / blood*,  drug therapy*
Lamivudine / therapeutic use*
Middle Aged
RNA, Viral / blood*
Reverse Transcriptase Inhibitors / therapeutic use*
Viral Load
Reg. No./Substance:
0/DNA, Viral; 0/Hepatitis B Core Antigens; 0/RNA, Viral; 0/Reverse Transcriptase Inhibitors; 134678-17-4/Lamivudine

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