| Hepatic uptake and metabolism of benzoate: a multiple indicator dilution, perfused rat liver study. | |
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MedLine Citation:
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PMID: 11352805 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multiple, noneliminated references ((51)Cr-labeled erythrocytes, (125)I-albumin, [(14)C]- or [(3)H]sucrose, and [(2)H](2)O), together with [(3)H]hippurate or [(14)C]benzoate, were injected simultaneously into the portal vein of the perfused rat liver during single-pass delivery of benzoate (5-1,000 microM) and hippurate (5 microM) to investigate hippurate formation kinetics and transport. The outflow dilution data best fit a space-distributed model comprising vascular and cellular pools for benzoate and hippurate; there was further need to segregate the cellular pool of benzoate into shallow (cytosolic) and deep (mitochondrial) pools. Fitted values of the membrane permeability-surface area products for sinusoidal entry of unbound benzoate were fast and concentration independent (0.89 +/- 0.17 ml. s(-1). g(-1)) and greatly exceeded the plasma flow rate (0.0169 +/- 0.0018 ml. s(-1). g(-1)), whereas both the influx of benzoate into the deep pool and the formation of hippurate occurring therein appeared to be saturable. Results of the fit to the dilution data suggest rapid uptake of benzoate, with glycination occurring within the deep and not the shallow pool as the rate-determining step. |
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Authors:
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A J Schwab; L Tao; T Yoshimura; A Simard; F Barker; K S Pang |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 280 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-15 Completed Date: 2001-06-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G1124-36 Citation Subset: IM |
Affiliation:
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McGill University Medical Clinic, Montreal General Hospital, Montreal, Quebec, Canada M5S 1A8. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzoates / metabolism*, pharmacokinetics* Glycine / metabolism Hippurates / metabolism Indicator Dilution Techniques Kinetics Liver / metabolism* Male Models, Biological Perfusion Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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GM-38250/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Benzoates; 0/Hippurates; 495-69-2/hippuric acid; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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