Document Detail

Hepatic and thymic alterations in newborn offspring of malnourished rat dams.
MedLine Citation:
PMID:  18407912     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Protein-energy malnutrition encompasses a group of conditions resulting from a lack of both protein and calories. It affects immune function and the gastrointestinal tract. Animal model studies of the effect of malnutrition during pregnancy and lactation have shown changes in the body weight of dams, with consequences for the pups. METHODS: Sixty healthy adult Wistar rats were mated for breeding. Pregnant dams (n = 19) were separated and randomly divided into 2 groups: normally nourished (NG; n = 10) and malnourished (MG; n = 9). The authors measured the average amount of feed eaten by the dams in the NG group in 24 hours and offered half this amount to the MG dams until delivery, after which both groups had an adequate amount of feed. Offspring were killed after birth and after lactation. Morphometric liver parameters were measured, and the tissue microarray method was performed manually to carry out histopathological and immunohistochemical analysis of thymuses from the offspring. RESULTS: Body weight, body length, and intestine length measured after birth were statistically lower (P < .05) in the MG group offspring than in the NG group offspring but recovered after lactation. Histological comparison of pups' livers revealed minimal differences. However, morphometric evaluation showed significant differences. Slides exposed to antivimentin and anti-CD45 RO stained positively in both groups, but the samples from the NG group were more heavily stained. CONCLUSIONS: Malnutrition in dams causes alterations in the liver, thymus, and other internal organs of newborn offspring. Significant changes in thymuses were found using human monoclonal antibodies that cross-react with rat proteins, a novel finding in the literature.
Sandra Lúcia Schuler; Jocemara Gurmini; William Augusto Casteleins Cecílio; Marina Luise Viola de Azevedo; Márcia Olandoski; Lúcia de Noronha
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  JPEN. Journal of parenteral and enteral nutrition     Volume:  32     ISSN:  0148-6071     ISO Abbreviation:  JPEN J Parenter Enteral Nutr     Publication Date:    2008 Mar-Apr
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-06-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7804134     Medline TA:  JPEN J Parenter Enteral Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  184-9     Citation Subset:  IM    
Experimental Pathology Laboratory, Center for Biological and Health Sciences, Pontifical Catholic University of Paraná, Curitiba, Paraná State, Brazil.
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MeSH Terms
Animals, Newborn / growth & development
Body Weight
Disease Models, Animal
Lactation / physiology*
Liver / pathology*
Microsomes, Liver / metabolism
Organ Size
Prenatal Nutritional Physiological Phenomena*
Protein Array Analysis
Protein-Energy Malnutrition / physiopathology*
Rats, Wistar
Thymus Gland / pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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