Document Detail


Hepatic stellate cells may be potential effectors of platelet activating factor induced portal hypertension.
MedLine Citation:
PMID:  18186558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To determine platelet activating factor (PAF) receptor expression in cirrhotic hepatic stellate cells. METHODS: Hepatic stellate cells, isolated from the livers of control and CCl(4)-induced cirrhotic rats, were placed in serum-free medium after overnight culture. We determined the PAF receptor in hepatic stellate cells by saturation binding technique and semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), and the effects of PAF and its antagonist BN52021 on prostaglandin E(2) (PGE(2)) release by stellate cells. RESULTS: Scatchard analysis indicated the presence of PAF receptor with dissociation constant (Kd) of 4.66 nmol/L and maximum binding capacity (Bmax) of 24.65 fmol/microg in cirrhotic stellate cells. Compared with the control, the maximum PAF binding capacity increased significantly (Bmax: 24.65 +/- 1.96 fmol/microg. DNA, R = 0.982 vs 5.74 +/- 1.55 fmol/microg. DNA, R = 0.93; P < 0.01), whereas receptor affinity had no significant difference (Kd of 4.66 +/- 0.33 nmol/L for the cirrhosis and 3.51 +/- 0.26 nmol/L for the control; P > 0.05). Consistent with the receptor binding data, the mRNA expression of PAF receptor was increased significantly in cirrhotic stellate cells. PAF in a concentration-dependent manner induced PGE(2) synthesis in cirrhotic hepatic stellate cells, but the effects were blocked significantly by BN52021. CONCLUSION: Cirrhosis sensitizes hepatic stellate cells to PAF by elevating its receptor level and hepatic stellate cells maybe potential effectors of PAF induced portal hypertension.
Authors:
Yan Chen; Chun-Ping Wang; Yin-Ying Lu; Lin Zhou; Shu-Hui Su; Hong-Jun Jia; Yong-Yi Feng; Yong-Ping Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  14     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-11     Completed Date:  2008-04-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  218-23     Citation Subset:  IM    
Affiliation:
Department of Gastroenterology, 302 Hospital of PLA, 100 Xisihuan Middle Road, Beijing 100039, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Hepatocytes / pathology,  physiology*
Hypertension, Portal / metabolism*,  pathology,  physiopathology
Liver Cirrhosis / metabolism*,  pathology
Male
Platelet Activating Factor / metabolism*
Platelet Membrane Glycoproteins / genetics,  metabolism*
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Platelet Activating Factor; 0/Platelet Membrane Glycoproteins; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 0/platelet activating factor receptor
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