Document Detail

Hepatic production of very low density lipoprotein, catabolism of low density lipoprotein, biliary lipid secretion, and bile salt synthesis in rats fed a bean (Phaseolus vulgaris) diet.
MedLine Citation:
PMID:  8509719     Owner:  NLM     Status:  MEDLINE    
Rats fed a bean diet develop a significant hypocholesterolemia. The catabolism of low density lipoprotein (LDL; d 1.019-1.063 g/ml) was studied in vivo and in vitro in the isolated perfused liver of rats fed either a casein or a bean diet. The clearance of LDL was significantly increased by 100% from 0.38 +/- 0.04 to 0.63 +/- 0.04 ml/h x 100 g body wt in vivo in the bean-fed rat. Similarly, the clearance of homologous and heterologous (human) LDL was also increased by 100% in the isolated perfused liver of bean-fed animals. Spleen, kidney, and hepatic cholesterogenesis was increased by 150% in these animals. Bile salt synthesis was increased from 1.54 +/- 0.02 to 2.84 +/- 0.09 nmol/min x g liver wt (P < 0.02) and biliary cholesterol output by 200% from 0.81 +/- 0.03 to 2.18 +/- 0.04 nmol/min x g (P < 0.02) in the isolated perfused liver of rats fed a bean diet. These results explained the depletion of hepatic cholesterol and were consistent with the LDL turnover studies, suggesting that apoB/E receptor activity was increased in these animals. ApoB and triglyceride secretion in the d < 1.060 g/ml lipoprotein fraction of liver perfusates remained normal in the bean-fed rats. In contrast, total sinusoidal cholesterol output isolated in the d < 1.060 g/ml fraction significantly decreased by 100% after 90 min of perfusion. Cholesterol output in the d > 1.060 g/ml lipoprotein fraction was unmodified by the bean diet. These data demonstrate that key metabolic pathways of hepatic cholesterol are modified in the bean-fed rat.(ABSTRACT TRUNCATED AT 250 WORDS)
M P Marzolo; L Amigo; F Nervi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  34     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-15     Completed Date:  1993-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  807-14     Citation Subset:  IM    
Departamento de Gastroenterología, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago.
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MeSH Terms
Apolipoproteins B / pharmacokinetics
Apolipoproteins E / pharmacokinetics
Bile / metabolism*
Bile Acids and Salts / metabolism
Cholesterol / metabolism*
Kidney / metabolism
Lipid Metabolism
Lipoproteins, LDL / pharmacokinetics
Lipoproteins, VLDL / metabolism
Liver / metabolism*
Plants, Medicinal*
Spleen / metabolism
Reg. No./Substance:
0/Apolipoproteins B; 0/Apolipoproteins E; 0/Bile Acids and Salts; 0/Lipoproteins, LDL; 0/Lipoproteins, VLDL; 57-88-5/Cholesterol

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