| Hepatic processing of cholecystokinin peptides. I. Structural specificity and mechanism of hepatic extraction. | |
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MedLine Citation:
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PMID: 3953818 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since the liver is a target tissue of many biologically important molecules, we have studied the hepatic uptake of cholecystokinin (CCK) with the isolated, perfused rat liver and a series of radioiodinated and unlabeled CCK peptides. Of the naturally occurring forms of CCK, cholecystokinin octapeptide (CCK-8) was extensively extracted (26 +/- 0.7% of Bolton-Hunter-labeled CCK-8, 59% of unlabeled CCK-8) in a single pass through the liver, while CCK-33 was minimally extracted (3.1 +/- 1.2% of Bolton-Hunter-labeled CCK-33). Studies of structural specificity showed that the sulfate ester on the tyrosine residue of CCK-8 decreased its hepatic extraction, that the carboxyl-terminal tetrapeptide region of CCK-8 was more important for this uptake process than was the amino-terminal tetrapeptide region, and that oxidation reduced uptake of CCK. First-pass hepatic extraction of unlabeled CCK-8 was shown to be a high-capacity process; however, uptake of radioiodinated CCK-8 was partially saturable with unlabeled CCK-4. Specific lectins (wheat-germ agglutinin, concanavalin A) and a bile acid (taurocholate) inhibited hepatic extraction of CCK-8 in a concentration-dependent manner. These data are consistent with a highly specific cellular extraction process for CCK in the liver. |
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Authors:
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G J Gores; N F LaRusso; L J Miller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 250 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-04-11 Completed Date: 1986-04-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G344-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cholecystokinin / metabolism* Iodine Radioisotopes / diagnostic use Liver / metabolism* Male Peptide Fragments / metabolism Perfusion Rats Rats, Inbred Strains Sincalide / analogs & derivatives, metabolism* Structure-Activity Relationship |
| Grant Support | |
ID/Acronym/Agency:
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AM-24031/AM/NIADDK NIH HHS; AM-32878/AM/NIADDK NIH HHS; AM-34988/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Iodine Radioisotopes; 0/Peptide Fragments; 25126-32-3/Sincalide; 80980-81-0/cholecystokinin (26-33); 9011-97-6/Cholecystokinin |
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