| Hepatic metabolism during constant infusion of fructose; comparative studies with 31P-magnetic resonance spectroscopy in man and rats. | |
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MedLine Citation:
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PMID: 8123666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A protocol of constant infusion of fructose has been carried out both in human volunteers and in the perfused rat liver, aiming at a steady-state blood fructose concentration of 6-8 mM. Localized 31P-NMR spectroscopy and biochemical analyses were used to evaluate the metabolic changes. Comparison of the model experiment and the clinical study allowed an evaluation of this protocol as a clinically relevant assessment of the metabolic function of the liver. The time course of change, as well as the quasi steady-state levels reached during fructose infusion, for phosphomonoesters (PME), ATP and inorganic phosphate (Pi) provided the following results: During fructose infusion, ATP and Pi reached a steady-state level of 74.0 +/- 5.9 and 54.6 +/- 3.3% of control respectively, in the human volunteers. The corresponding data in the rat liver was 71.3 +/- 4.3 and 54.4 +/- 4.3%. Hepatic clearance of fructose was 0.53 and 0.52 ml.g liver-1.min-1 for volunteers and rats, respectively. The time course of intracellular metabolite recovery after fructose could be approximated by a first order kinetic. The rate constants for PME and ATP change were similar during fructose infusion and recovery, while after the discontinuation of fructose infusion, Pi increased with a rate constant significantly greater than during its fructose-induced depletion in human liver (P < 0.005). Thus, this relatively simple clinically applicable protocol seems to be verifiable in the well controlled perfused rat liver model, and it is argued that it may be useful in the clinical evaluation of the metabolic functional capacity of the human liver. |
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Authors:
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S Masson; O Henriksen; A Stengaard; C Thomsen; B Quistorff |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1199 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1994 Mar |
Date Detail:
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Created Date: 1994-04-12 Completed Date: 1994-04-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 166-74 Citation Subset: IM |
Affiliation:
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NMR Centre, Dept. of Medical Biochemistry and Genetics, Copenhagen N, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Fructose / administration & dosage*, metabolism Gluconeogenesis Glycolysis Humans Infusions, Intravenous Liver / metabolism* Magnetic Resonance Spectroscopy Male Perfusion Phosphorylation Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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30237-26-4/Fructose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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