| Hepatic metabolism of diallyl disulphide in rat and man. | |
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MedLine Citation:
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PMID: 14742141 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The metabolism of diallyl disulphide was investigated in vitro with rat and human liver cell subfractions and ex vivo with an isolated perfused rat liver. 2. Diallyl disulphide was oxidized to diallylthiosulphinate by rat liver microsomes with an apparent K(m) = 0.86 +/- 0.1 mM and an apparent V(max) = 0.47 +/- 0.12 nmol min(-1) mg(-1) protein (mean +/- SE). Both cytochrome P450 (CYP) and flavin-containing monooxygenases were involved, with CYP2B1/2 and CYP2E1 being the most active CYP enzymes. 3. In rat and man, microsomal oxidation of allylmethyl sulphide to allylmethyl sulphoxide and allylmethyl sulphone also occurred, although at a low rate. Diallyl disulphide was also metabolized to allylglutathione sulphide and allylmercaptan. In addition, diallylthiosulphinate reacted non-enzymatically with glutathione to form allylglutathione sulphide. 4. When an isolated rat liver was perfused with diallyl disulphide, the metabolites allyl mercaptan, allylmethyl sulphide, allylmethyl sulphoxide, allylmethyl sulphone and allylglutathione sulphide were detected primarily within the liver tissue, with only small amounts of metabolites found in the bile and perfusion medium. The pharmacokinetic parameters for diallyl disulphide were t(1/2) = 6.09 min; AUC(0- infinity ) = 4.77 min mmol l(-1); clearance = 34.22 ml min(-1). 5. A scheme for the metabolism of diallyl disulphide in rat and man is proposed. |
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Authors:
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E Germain; J Chevalier; M-H Siess; C Teyssier |
Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Xenobiotica; the fate of foreign compounds in biological systems Volume: 33 ISSN: 0049-8254 ISO Abbreviation: Xenobiotica Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2004-01-26 Completed Date: 2004-11-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1306665 Medline TA: Xenobiotica Country: England |
Other Details:
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Languages: eng Pagination: 1185-99 Citation Subset: IM |
Affiliation:
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Unité Mixte de Recherche de Toxicologie Alimentaire, Institut National de Recherche Agronomique--Université de Bourgogne, Dijon Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Allyl Compounds / metabolism* Animals Area Under Curve Aryl Hydrocarbon Hydroxylases / metabolism Cytochrome P-450 CYP2B1 / metabolism Cytochrome P-450 CYP2E1 / metabolism Disulfides / metabolism* Enzymes / metabolism* Female Humans Liver / metabolism* Male Microsomes, Liver / metabolism Middle Aged Oxidation-Reduction Perfusion Rats Steroid Hydroxylases / metabolism Sulfur Compounds / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Allyl Compounds; 0/Disulfides; 0/Enzymes; 0/Sulfur Compounds; 2179-57-9/diallyl disulfide; EC 1.14.-/Steroid Hydroxylases; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Cytochrome P-450 CYP2B1; EC 1.14.14.1/Cytochrome P-450 CYP2E1; EC 1.14.14.1/steroid 16-beta-hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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