| Hepatic lacI and cII mutation in transgenic (lambdaLIZ) rats treated with dimethylnitrosamine. | |
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MedLine Citation:
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PMID: 9804924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The recent introduction of a transgenic rat in vivo mutation assay is a much needed supplement to the transgenic mouse models and offers the tools necessary for collecting target tissue specific genotoxicity data in this species. The utility of the Big Blue(R) rat for the detection of in vivo mutations was investigated by studying spontaneous and dimethylnitrosamine (DMN)-induced hepatic mutations. High molecular weight DNA isolated from Big Blue(R) rat livers typically yielded good transgene rescue efficiency of up to 5x105 plaque forming units per packaging reaction. DMN, when administered by oral gavage at dose levels of 0.2, 0.6, 2.0, and 6.0 mg kg-1 day-1, induced up to a 4.5-fold increase in mutations at the highest dose level. There was no apparent difference between the lacI vs. cII target genes of the shuttle vector in either the background or DMN-induced mutant frequencies. These results suggest that the transgenic rat model is a useful tool for studying potential genotoxicity in target organs and, with further validation, the selectable cII target could be an attractive alternative to the conventional lacI color screening method for the detection of mutations in the lambdaLIZ shuttle vector. |
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Authors:
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B B Gollapudi; K M Jackson; W T Stott |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mutation research Volume: 419 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1998-12-15 Completed Date: 1998-12-15 Revised Date: 2006-03-17 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 131-5 Citation Subset: IM |
Copyright Information:
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Copyright 1998 Elsevier Science B.V. |
Affiliation:
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The Dow Chemical, Health and Environmental Research Laboratory, 1803 Building, Midland, MI 48674, USA. bgollapudi@dow.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified* Bacteriophage lambda / genetics Dimethylnitrosamine / toxicity* Lactose Factors / genetics* Liver / ultrastructure Mutagenicity Tests* Mutation* Rats Rats, Inbred F344 Transcription Factors / genetics* Viral Proteins |
| Chemical | |
Reg. No./Substance:
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0/Transcription Factors; 0/Viral Proteins; 0/cII protein, bacteriophage lambda; 62-75-9/Dimethylnitrosamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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