Document Detail


Hepatic in vitro toxicity assessment of PBDE congeners BDE47, BDE153 and BDE154 in Atlantic salmon (Salmo salar L.).
MedLine Citation:
PMID:  21767471     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The brominated flame retardant congeners BDE47, BDE153 and BDE154 are among the congeners accumulating to the highest degree in fish. In order to gain knowledge about the toxicological effects of PBDEs in fish, microarray-based transcriptomic and 2D-DIGE/MALDI-TOF/TOF proteomic approaches were used to screen for effects in primary Atlantic salmon hepatocytes exposed to these congeners alone or in combination (PBDE-MIX). A small set of stress related transcripts and proteins were differentially expressed in the PBDE exposed hepatocytes. The PBDE-MIX, and BDE153 to a lesser degree, seems to have induced metabolic disturbances by affecting several pathways related to glucose homeostasis. Further, effects on cell cycle control and proliferation signal pathways in PBDE-MIX-exposed hepatocytes clearly suggest that the PBDE exposure affected cell proliferation processes. CYP1A was 7.41- and 7.37-fold up-regulated in hepatocytes exposed to BDE47 and PBDE-MIX, respectively, and was the only biotransformation pathway affected by the PBDE exposure. The factorial design and PLS regression analyses of the effect of the PBDE-MIX indicated that BDE47 contributed the most to the observed CYP1A response, suggesting that this congener should be incorporated in the toxic equivalent (TEQ) concept in future risk assessment of dioxin-like chemicals. Additionally, a significant up-regulation of the ER-responsive genes VTG and ZP3 was observed in cells exposed to BDE47 and PBDE-MIX. Further analyses suggested that BDE47 and BDE154 have an estrogenic effect in male fish. The data also suggested an antagonistic interaction between BDE153 and BDE154. In conclusion, this study shows that PBDEs can affect several biological systems in Atlantic salmon cells, and demonstrates the need for more studies on the simultaneous exposure to chemical mixtures to identify combined effects of chemicals.
Authors:
Liv Søfteland; Kjell Petersen; Anne-Kristin Stavrum; Terence Wu; Pål A Olsvik
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-31
Journal Detail:
Title:  Aquatic toxicology (Amsterdam, Netherlands)     Volume:  105     ISSN:  1879-1514     ISO Abbreviation:  Aquat. Toxicol.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-11-02     Completed Date:  2012-07-27     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  8500246     Medline TA:  Aquat Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  246-63     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
National Institute of Nutrition and Seafood Research, PO Box 2029 Nordnes, N-5817 Bergen, Norway. lso@nifes.no
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MeSH Terms
Descriptor/Qualifier:
Animals
Electrophoresis, Gel, Two-Dimensional
Fish Proteins / drug effects
Flame Retardants / toxicity*
Gene Expression Profiling
Hepatocytes / drug effects*,  metabolism
Male
Polybrominated Biphenyls / toxicity*
Protein Array Analysis
Proteome / drug effects*
Real-Time Polymerase Chain Reaction
Regression Analysis
Salmo salar / genetics,  metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Toxicity Tests, Chronic
Transcriptome / drug effects*
Water Pollutants, Chemical / toxicity*
Chemical
Reg. No./Substance:
0/Fish Proteins; 0/Flame Retardants; 0/Polybrominated Biphenyls; 0/Proteome; 0/Water Pollutants, Chemical; 0/hexabrominated diphenyl ether 153; 0/hexabromodiphenyl ether 154; 0N97R5X10X/2,2',4,4'-tetrabromodiphenyl ether

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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