| Hepatic disposition and toxicity of cationized goat immunoglobulin G and fab fragments in isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 9660848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Colchicine-specific goat IgG and Fab fragments were cationized by covalent coupling of hexamethylenediamine. The immunoreactivity of antibodies was not changed following cationization. The interaction of 125I-radiolabeled native (nIgG and nFab) and cationized immunoglobulin G (cIgG) and Fab fragments (cFab) with liver was investigated using isolated perfused rat liver (IPRL) and isolated rat hepatic parenchymal cells (PCs) and nonparenchymal cells (NPCs) in suspension. 125I-cIgG or 125I-cFab were more rapidly cleared from the perfusate than the corresponding native proteins. Both cIgG and cFab declined biexponentially over time in the perfusate. In contrast, the native IgG and Fab decreased monoexponentially. The half-lives of the initial and terminal phases were 5.2 +/- 1.6 min and 355.1 +/- 17.2 min for cIgG and 14.7 +/- 3.4 min and 552.4 +/- 23.7 min for cFab. The terminal half-lives of nIgG (467.4 +/- 11.6 min) and nFab (880.1 +/- 39.6 min) were longer than those of cationized molecules. The biliary protein extraction ratio of cationized IgG and Fab was greater than that of native IgG and Fab: 0.13% (cIgG), 0.02% (nIgG), 0.23% (cFab), and 0.17% (nFab). The uptake of cIgG and cFab by both PCs and NPCs was dose-dependent and was about 6-fold and 8-fold higher than that of their native counterparts, respectively. Throughout the experiment, liver viability was determined, and no toxicity was observed according to physiological analysis (bile flow rate, portal vein pressure, and pH) and biochemical analysis (glucose and hepatic enzymes: alanine transaminase, aspartate transaminase, lactate dehydrogenase) in perfusate. |
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Authors:
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G Hong; M Bazin-Redureau; P Gires; J M Scherrmann |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Drug metabolism and disposition: the biological fate of chemicals Volume: 26 ISSN: 0090-9556 ISO Abbreviation: Drug Metab. Dispos. Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-08-24 Completed Date: 1998-08-24 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9421550 Medline TA: Drug Metab Dispos Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 661-9 Citation Subset: IM |
Affiliation:
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INSERM U26 Département de Pharmacocinétique, Hôpital Fernad, 75475 Paris, CEDEX 10, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Erythrocytes / metabolism Goats Immunoglobulin Fab Fragments / metabolism*, toxicity Immunoglobulin G / metabolism*, toxicity Liver / metabolism* Male Perfusion Rats Rats, Sprague-Dawley Serum Albumin, Bovine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin Fab Fragments; 0/Immunoglobulin G; 0/Serum Albumin, Bovine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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