Document Detail


Hepatic disposition characteristics of recombinant human interleukin-11 (rhIL-11) in the perfused rat liver.
MedLine Citation:
PMID:  9881656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hepatic disposition characteristics of recombinant human interleukin-11 (rhIL-11) were investigated in perfused rat liver to clarify the mechanism of hepatic clearance which is a major contributor to the rapid clearance of rhIL-11 in vivo. We analyzed the disposition characteristics of [(111)In]-labeled rhIL-11 using a single-pass constant infusion mode at different concentrations of rhIL-11. The venous outflow rapidly reached a steady-state condition at every concentration. Liver extraction ratio at steady-state (Ess) was decreased with increase in the concentration, suggesting that there is a saturable interaction between the liver cells and rhIL-11 molecule. Cellular localization experiments demonstrated that rhIL-11 was taken up by both liver parenchymal and nonparenchymal cells depending on their surface area, suggesting that this uptake was mediated by electrostatic interaction due to cationic charges in the cytokine.
Authors:
A Takagi; Y Yabe; M Yoshida; Y Takakura; M Hashida
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  21     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-23     Completed Date:  1999-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1364-6     Citation Subset:  IM    
Affiliation:
Novel Pharmaceutical Laboratories, Yamanouchi Pharmaceutical Co., Ltd., Yaizu, Shizuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Humans
Interleukin-11 / pharmacokinetics*
Liver / metabolism*
Male
Perfusion
Rats
Rats, Wistar
Recombinant Proteins / pharmacokinetics
Chemical
Reg. No./Substance:
0/Interleukin-11; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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