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HEPATIC DIFFERENTIATION OF MOUSE ES CELLS AND IPS CELLS DURING ORGANOID FORMATION IN HOLLOW FIBERS.
MedLine Citation:
PMID:  21457096     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We have focused on pluripotent stem cells as a potential source of a hybrid-type artificial liver (HAL) and tried to develop a method for differentiating the pluripotent stem cells into cells of a hepatic lineage. In this study, we investigated the hepatic differentiation of mouse embryonic stem (ES) cells and induced pluripotent stem (iPS) cells by the applying hollow fiber (HF)/organoid culture method, in which cultured cells form a cellular aggregate called an "organoid" in the lumen of the HF. ES and iPS cells were injected into HFs to induce organoid formation, and cells were cultured. To induce hepatic differentiation, we added differentiation-promoting agents to the culture medium. The expression levels of differentiation-related genes were up-regulated, with cell proliferation and organoid formation inside HFs. Because we were able to achieve a high cell density in culture, the maximum levels of liver-specific functions per unit volume in the differentiating ES and iPS cells reached a level comparable to or better than that of primary mouse hepatocytes. In conclusion, ES and iPS cells have the potential to be a cell source for a HAL, and the HF/organoid culture method has promise as a basic technology for the development of a HAL.
Authors:
Naoki Amimoto; Hiroshi Mizumoto; Kohji Nakazawa; Hiroyuki Ijima; Kazumori Funatsu; Toshihisa Kajiwara
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-2
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Kyushu University, Department of Chemical Engineering, Faculty of Engineering, 744 Motooka, Nishi-ku, Fukuoka, Japan, 819-0395, +81-92-802-2776, +81-92-802-2796; namimoto@chem-eng.kyushu-u.ac.jp.
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