Document Detail


Hepatic clearance of Sonazoid perfluorobutane microbubbles by Kupffer cells does not reduce the ability of liver to phagocytose or degrade albumin microspheres.
MedLine Citation:
PMID:  12712317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study has been performed to examine which cells are responsible for the hepatic clearance of the new ultrasound contrast agent Sonazoid and to study whether uptake of these gas microbubbles disturbs the function of the cells involved. Sonazoid was injected into rats and perfused fixed livers were studied by electron microscopy, which revealed that the Sonazoid microbubbles were exclusively internalised in Kupffer cells, i.e. by the macrophages located in the liver sinusoids, and not by parenchymal, stellate or endothelial cells. This is the first demonstration of intact phagocytosed gas microbubbles within Kupffer cells. Uptake of the Sonazoid perfluorobutane microbubbles by the Kupffer cells following injection of a dose corresponding to 20x the anticipated clinical dose for liver imaging did not result in measurable changes in the uptake and degradation of radioactively labelled albumin microspheres previously shown to be a useful indicator marker for Kupffer cell phagocytosis.
Authors:
Grete Mørk Kindberg; Helge Tolleshaug; Norbert Roos; Tore Skotland
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Publication Detail:
Type:  Journal Article     Date:  2003-02-27
Journal Detail:
Title:  Cell and tissue research     Volume:  312     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-24     Completed Date:  2004-02-27     Revised Date:  2011-12-12    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  49-54     Citation Subset:  IM    
Affiliation:
Research and Development, Amersham Health AS, P.O. Box 4220 Nydalen, N-0401, Oslo, Norway. grete.mork.kindberg@amersham.com
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MeSH Terms
Descriptor/Qualifier:
Albumins / metabolism*
Animals
Contrast Media / metabolism*
Ferric Compounds / metabolism*
Iron / metabolism*
Kupffer Cells / metabolism*,  ultrastructure
Liver / cytology,  metabolism*
Microspheres
Oxides / metabolism*
Phagocytosis / physiology*
Random Allocation
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Albumins; 0/Contrast Media; 0/Ferric Compounds; 0/Oxides; 0/Sonazoid; 7439-89-6/Iron

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