| Hepatic Kupffer cell phagocytotic function in rats with erythrocytic-stage malaria. | |
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MedLine Citation:
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PMID: 12084035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In the erythrocytic phase of malaria, Kupffer cells show marked hypertrophy and hyperplasia and are filled with malarial pigment. However, phagocytic function in this state has not been well characterized. The aim of the present study was to use mouse Plasmodium berghei to infect rats with malaria and study the phagocytic function and morphology of Kupffer cells. METHODS: We used a recirculating isolated perfused rat liver (IPRL) to quantitate Kupffer cell phagocytic clearance of radiolabeled albumin-latex over 120 min in high parasitemia (53 +/- 6%; n = 7) and low parasitemia (approximately 1%; n = 4) malaria-infected rats and littermate controls (n = 7 and n = 4, respectively). In a further group of high-parasitemic rats, perfusion was ceased after 7 min and liver radioactivity also measured. Electron microscopy was performed after perfusions. RESULTS: In high-parasitemia malaria rats, clearance of radiolabeled latex from IPRL perfusate over 120 min was significantly (P < 0.01) faster than in controls, with a lower area under the curve (0.19 +/- 0.02 vs 0.43 +/- 0.07 /mL per min, respectively) and shorter half-life (t1/2k; 2.4 +/- 0.6 vs 10.0 +/- 2.3 min, respectively). Low-parasitemia rats were identical to controls. After 7 min perfusion in high-parasitemic rats (n = 4), total radioactivity in liver homogenates was higher than in controls (n = 4; 33.1 +/- 6.2 vs 18.4 +/- 1.9% of injected radiolabel; P < 0.05). Electron microscopy showed latex in Kupffer cells, more abundantly seen in high-parasitemic animals. CONCLUSIONS: Total Kupffer cell phagocytic activity of the liver is markedly increased in rats with a high parasitemic load of malarial P. berghei infection. This is presumed to reflect an upregulation of scavenger activity phagocytosing erythrocytes and their breakdown products. |
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Authors:
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Michael S Nobes; Hany Ghabrial; Katrina M Simms; Richard B Smallwood; Denis J Morgan; Richard B Sewell |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of gastroenterology and hepatology Volume: 17 ISSN: 0815-9319 ISO Abbreviation: J. Gastroenterol. Hepatol. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-06-26 Completed Date: 2002-09-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: Australia |
Other Details:
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Languages: eng Pagination: 598-605 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Blackwell Publishing Asia Pty Ltd |
Affiliation:
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Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Erythrocytes / physiology* Hemolysis* Kupffer Cells / physiology* Malaria / blood*, parasitology, pathology, physiopathology* Male Mice Mice, Inbred BALB C Microscopy, Electron Parasitemia / parasitology Perfusion Phagocytosis* Rats Rats, Wistar |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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