Document Detail


Heparin-mediated transformation of Escherichia coli with Ustilago maydis DNA.
MedLine Citation:
PMID:  1878203     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The inhibiton of Escherichia coli transformation caused by simple preparations of Ustilago maydis plasmid and genomic DNA can be efficiently overcome by the use of the linear polyanion heparin. Techniques using heparin have been derived that should facilitate the production and isolation of plasmids passed through Ustilago maydis and may be of benefit in other systems where similar inhibitory phenomena or low transformability are observed.
Authors:
R P Bauchwitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioTechniques     Volume:  10     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  710-8     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Cornell University Medical College, New York, NY 10021.
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / genetics*
Genes, Fungal / genetics
Heparin / pharmacology*
Plasmids
Transformation, Genetic*
Ustilago / genetics*
Chemical
Reg. No./Substance:
9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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