| Heparin inhibits the production of matrix metalloproteinase-2 and improves atherosclerosis in LDL receptor-deficient mice. | |
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MedLine Citation:
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PMID: 19940766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: This study aimed to find the effects of heparin on atherosclerosis and the production of matrix metalloproteinase (MMP)-2 in low-density lipoprotein receptor-deficient (LDLr(-/-)) mice. METHODS: Sixteen 7-week-old LDLr(-/-) mice were randomized to receive sterile water or heparin. The levels of total cholesterol, high-density lipoprotein cholesterol, triglyceride and homocysteine were measured. Mean lesions area was calculated as the total atherosclerotic lesions area and expressed as a percentage of total luminal surface area. The lesions area was measured blindly by the same person using computer-assisted image analysis. The expression and localization of the MMP-2 was examined by immunohistochemistry. RESULTS: All mice exhibited atherosclerotic lesions in the aortic sinus and aortic surface. Total cholesterol was decreased, while high-density lipoprotein cholesterol was increased in heparin compared with that in control group (P=0.001 and 0.002). Triglyceride was not significantly different between the two groups (P=0.92). The amount of atherosclerotic lesions in the aortic surface was 40.5% lower in heparin group than that in the control group (P<0.001). The mean area of atherosclerotic lesions in the aortic sinus was also less in the heparin group than that in the control group. Coincidently, the expression of MMP-2 in the atherosclerotic lesions in the heparin group was 49.3% lower than that in the control group (P<0.001). CONCLUSION: Heparin can inhibit the production of MMP-2 in the atherosclerotic lesions and improve the atherosclerotic lesions in LDLr(-/-) mice. |
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Authors:
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Hangyuan Guo; Fukang Xu; Aijing Sun; Longbin Liu; Yafei Shi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Coronary artery disease Volume: 21 ISSN: 1473-5830 ISO Abbreviation: Coron. Artery Dis. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-22 Completed Date: 2010-03-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9011445 Medline TA: Coron Artery Dis Country: England |
Other Details:
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Languages: eng Pagination: 39-45 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Shaoxing People's Hospital, Zhejiang, China. ghangyuan@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / drug effects*, enzymology, pathology Atherosclerosis / drug therapy*, enzymology, pathology Biological Markers / blood Body Weight Cardiovascular Agents / pharmacology* Cholesterol / blood Cholesterol, HDL / blood Disease Models, Animal Eating Heparin / pharmacology* Homocysteine / blood Immunohistochemistry Male Matrix Metalloproteinase 2 / metabolism* Mice Mice, Inbred C57BL Mice, Knockout Receptors, LDL / deficiency*, genetics Triglycerides / blood |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Cardiovascular Agents; 0/Cholesterol, HDL; 0/Receptors, LDL; 0/Triglycerides; 454-28-4/Homocysteine; 57-88-5/Cholesterol; 9005-49-6/Heparin; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.24/Mmp2 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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