Document Detail


Heparin-inhibitable lectins: marked similarities in chicken and rat.
MedLine Citation:
PMID:  7299837     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Extracts of young rat lung contain a heparin-inhibitable lectin that closely resembles one recently purified from chicken liver. Both lectins interact with heparin and N-acetyl-D-galactosamine, and were purified by gel filtration on Sepharose CL-2B followed by affinity chromatography on heparin-Sepharose. They both behave as high molecular weight aggregates that can be dissociated into two peptides with apparent molecular weights of 13,000 and 16,000 by gel electrophoresis in SDS. Samples of purified lectin contained up to 20% DNA by weight, and the degree of lectin aggregation and hemagglutination activity was greatly reduced by treatment with micrococcal nuclease without inhibiting heparin-binding activity. Association of lectin with DNA is an artifact of homogenization in high salt, since only 2% of the lectin is found associated with a purified nuclear fraction.
Authors:
M M Roberson; H Ceri; P J Shadle; S H Barondes
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of supramolecular structure and cellular biochemistry     Volume:  15     ISSN:  0275-3723     ISO Abbreviation:  J Supramol Struct Cell Biochem     Publication Date:  1981  
Date Detail:
Created Date:  1982-01-09     Completed Date:  1982-01-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8106911     Medline TA:  J Supramol Struct Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  395-402     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chickens
DNA / isolation & purification
Heparin / pharmacology*
Lectins / isolation & purification*
Liver / analysis
Lung / analysis
Male
Molecular Weight
Rats
Species Specificity
Chemical
Reg. No./Substance:
0/Lectins; 9005-49-6/Heparin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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