| Heparin increased cell membrane-associated heparan sulfate proteoglycan in balloon-injured rat carotid artery. | |
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MedLine Citation:
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PMID: 8518334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of heparin on the amount of membrane-associated heparan sulfate proteoglycan (HSPG) was examined biochemically in balloon-injured rat carotid arteries. Heparin (Choay 1772) was administered at the rate of 1 mg/kg/h.i.v. by an osmotic infusion pump for 10 days. Arterial tissues were incubated ex vivo with Na2(35)SO4 and 3H-glucosamine for 6 or 24 h. The arteries from heparin-treated rats were also incubated with heparin (100 micrograms/ml) during the radiolabeling. Membrane-associated proteoglycans were extracted with 4 M guanidine-HCl and isolated by a series of chromatographic steps using Sephadex G-50, DEAE-Sephacel and Octyl-Sepharose CL-4B. Membrane-associated proteoglycans were eluted with 0.8% Triton X-100 from Octyl-Sepharose columns. Heparin treatment significantly increased membrane-associated HSPG by 64% in the arteries incubated for 24 h, while heparin hardly increased the HSPG in 6 h incubated arteries. Since the 24-hour data seem to reflect a combination of biosynthesis and degradation of proteoglycans while the 6-hour data primarily reflect biosynthesis, these results suggest that inhibition of degradation of membrane-associated HSPG is involved in the mechanism of heparin action. This speculation is supported by the observation that membrane-associated HSPG was elevated in balloon-injured arteries by treatment with protease inhibitors. |
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Authors:
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S Mutoh; M M Clowes; A W Clowes |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of vascular research Volume: 30 ISSN: 1018-1172 ISO Abbreviation: J. Vasc. Res. Publication Date: 1993 May-Jun |
Date Detail:
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Created Date: 1993-07-28 Completed Date: 1993-07-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9206092 Medline TA: J Vasc Res Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 161-8 Citation Subset: IM |
Affiliation:
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Department of Surgery, School of Medicine, University of Washington, Seattle. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Balloon Dilatation* Carotid Arteries / metabolism* Carotid Artery Injuries* Cell Membrane / metabolism Chromatography, Agarose Heparan Sulfate Proteoglycans Heparin / pharmacology* Heparitin Sulfate / metabolism* Protease Inhibitors / pharmacology Proteoglycans / metabolism* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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HL18645/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Heparan Sulfate Proteoglycans; 0/Protease Inhibitors; 0/Proteoglycans; 9005-49-6/Heparin; 9050-30-0/Heparitin Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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