| Heparin and aspirin attenuate placental apoptosis in vitro: implications for early pregnancy failure. | |
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MedLine Citation:
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PMID: 15671997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Live birth rates are increased by treatment with heparin and aspirin in cases of poor pregnancy outcome such as antiphospholipid syndrome. Both drugs may attenuate miscarriage by inhibiting aberrant coagulation or by modulating trophoblast apoptosis. Here we assessed their roles in trophoblast apoptosis in vitro. STUDY DESIGN: BeWo cells and placental villi were cultured in sera from women with successful or failing in vitro fertilization, with and without heparin or aspirin. Apoptosis was assessed by using DNA laddering, cytokeratin 18 neoepitope formation, Bcl-2, and caspase 7 expression. RESULTS: In BeWo cells, sera from in vitro fertilization failure increased trophoblast apoptosis, whereas heparin and aspirin reversed these effects. In villous trophoblast, heparin increased Bcl-2 and cytokeratin 18 protein expression. Heparin and aspirin inhibited DNA laddering. CONCLUSION: Heparin and aspirin modulate trophoblast apoptosis suggesting a direct impact on trophoblast biology, thus providing an additional mechanism to explain the clinical benefits of heparin and aspirin on recurrent pregnancy loss. |
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Authors:
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Patrick Bose; Simon Black; Mamed Kadyrov; Ute Weissenborn; Joseph Neulen; Lesley Regan; Berthold Huppertz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 192 ISSN: 0002-9378 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-26 Completed Date: 2005-03-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 23-30 Citation Subset: AIM; IM |
Affiliation:
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Department of Anatomy II, University Hospital RWTH, Aachen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abortion, Habitual
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pathology,
prevention & control Anticoagulants / administration & dosage, pharmacology*, therapeutic use Apoptosis Aspirin / administration & dosage, pharmacology*, therapeutic use Cell Line / drug effects Dose-Response Relationship, Drug Female Fertilization in Vitro Heparin / administration & dosage, pharmacology*, therapeutic use Humans Placenta / drug effects* Placenta Diseases / pathology, prevention & control* Pregnancy Pregnancy Complications, Hematologic / pathology, prevention & control* Trophoblasts / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 50-78-2/Aspirin; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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