| Heparin affects matrix metalloproteinases and tissue inhibitors of metalloproteinases circulating in peripheral blood. | |
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MedLine Citation:
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PMID: 18926810 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Blood sampling/handling alters matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) expression. The aim of this study is to evaluate the effects of high molecular weight heparin on MMP and TIMP expression in blood. DESIGN AND METHODS: We analyzed by gelatin zymography and ELISA assays the effects of different heparin salts, dose- and time-dependence of MMP and TIMP concentrations in plasma and sera collected with and without clot-accelerator in plastic tubes from 50 healthy donors. RESULTS: The levels and zymography of MMP-2 did not show significant changes among all samples, and during time- and dose-dependent heparin treatments. MMP-9 and TIMP-2 expression were strongly affected by heparin, with significant increase of their content and gelatinolytic activity both in time- and in dose-dependent fashion. Addition of heparin allowed also the displacement of MMP-2 prodomain, favouring zymogen activation. CONCLUSIONS: Heparin has direct and indirect effects, altering MMP/TIMP complexes circulating in blood, and increasing the release of TIMP-2. To avoid misinterpretations due to MMP/TIMP complex alteration and MMP prodomain displacement, heparin should be cautiously used in blood collection procedures. |
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Authors:
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Ferdinando Mannello; Klaus Jung; Gaetana A Tonti; Franco Canestrari |
Publication Detail:
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Type: Journal Article Date: 2008-10-02 |
Journal Detail:
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Title: Clinical biochemistry Volume: 41 ISSN: 1873-2933 ISO Abbreviation: Clin. Biochem. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0133660 Medline TA: Clin Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 1466-73 Citation Subset: IM |
Affiliation:
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Department of Biomolecular Sciences, Section of Clinical Biochemistry, University Carlo Bo, Urbino, Italy. ferdinando.mannello@uniurb.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Blood / drug effects*, metabolism Blood Specimen Collection Dose-Response Relationship, Drug Enzyme Activation Enzyme-Linked Immunosorbent Assay Female Heparin / pharmacology* Humans Male Matrix Metalloproteinase 2 / metabolism* Matrix Metalloproteinase 9 / metabolism* Middle Aged Tissue Inhibitor of Metalloproteinase-1 / metabolism* Tissue Inhibitor of Metalloproteinase-2 / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Tissue Inhibitor of Metalloproteinase-1; 127497-59-0/Tissue Inhibitor of Metalloproteinase-2; 9005-49-6/Heparin; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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