Document Detail

Heparin-binding EGF-like growth factor protects pericytes from injury.
MedLine Citation:
PMID:  20863525     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: We have previously shown that heparin-binding EGF-like growth factor (HB-EGF) promotes angiogenesis and preserves mesenteric microvascular blood flow in several models of intestinal injury. The current study was designed to evaluate the effect of HB-EGF on pericytes, since these cells function to regulate capillary blood flow and new capillary growth.
MATERIALS AND METHODS: C3H/10T1/2 mouse mesenchymal cells were differentiated into pericyte-like cells in vitro using transforming growth factor-β1 (TGF-β1). In addition, primary pericyte cultures were established from rat brain. The effect of HB-EGF on pericyte proliferation was assessed. In addition, cells were stressed by exposure to anoxia, and apoptosis determined. In vivo, we examined the effect of HB-EGF on pericytes in a model of intestinal I/R injury based on superior mesenteric artery occlusion (SMAO) in mice.
RESULTS: Differentiated C3H/10T1/2 cells (pericyte-like cells) demonstrated morphologic characteristics of pericytes, and expressed pericyte specific markers. Addition of HB-EGF led to significant cell proliferation in differentiated pericyte-like cells, even under conditions of anoxic stress. Addition of the EGF receptor inhibitor AG 1478 led to complete inhibition of the proliferative effects of HB-EGF on pericyte-like cells. In addition, HB-EGF protected pericyte-like cells from anoxia-induced apoptosis. In addition, HB-EGF promoted cell proliferation in primary pericyte cultures. In vivo, administration of HB-EGF to mice subjected to intestinal I/R injury led to protection of pericytes from injury.
CONCLUSIONS: These results suggest that HB-EGF may function as a microcirculatory blood flow regulator, at least in part, via its effects on pericytes.
Xiaoyi Yu; Andrei Radulescu; Chun-Liang Chen; Iyore O James; Gail E Besner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-17
Journal Detail:
Title:  The Journal of surgical research     Volume:  172     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-14     Completed Date:  2012-02-14     Revised Date:  2013-04-15    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-76     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Department of Pediatric Surgery, Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, The Ohio State University College of Medicine, Columbus, Ohio, USA.
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MeSH Terms
Anoxia / physiopathology
Apoptosis / drug effects*,  physiology
Cell Differentiation / drug effects
Cell Line
Cell Proliferation / drug effects*
Cells, Cultured
Intercellular Signaling Peptides and Proteins / pharmacology*
Intestines / blood supply
Mesoderm / cytology,  drug effects
Mice, Inbred C3H
Models, Animal
Pericytes / cytology*,  drug effects*,  physiology
Rats, Sprague-Dawley
Reperfusion Injury / pathology,  physiopathology,  prevention & control
Transforming Growth Factor beta1 / pharmacology
Grant Support
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Transforming Growth Factor beta1; 149176-25-0/heparin-binding EGF-like growth factor

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