Document Detail


Heparin-binding EGF-like growth factor protects pericytes from injury.
MedLine Citation:
PMID:  20863525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: We have previously shown that heparin-binding EGF-like growth factor (HB-EGF) promotes angiogenesis and preserves mesenteric microvascular blood flow in several models of intestinal injury. The current study was designed to evaluate the effect of HB-EGF on pericytes, since these cells function to regulate capillary blood flow and new capillary growth.
MATERIALS AND METHODS: C3H/10T1/2 mouse mesenchymal cells were differentiated into pericyte-like cells in vitro using transforming growth factor-β1 (TGF-β1). In addition, primary pericyte cultures were established from rat brain. The effect of HB-EGF on pericyte proliferation was assessed. In addition, cells were stressed by exposure to anoxia, and apoptosis determined. In vivo, we examined the effect of HB-EGF on pericytes in a model of intestinal I/R injury based on superior mesenteric artery occlusion (SMAO) in mice.
RESULTS: Differentiated C3H/10T1/2 cells (pericyte-like cells) demonstrated morphologic characteristics of pericytes, and expressed pericyte specific markers. Addition of HB-EGF led to significant cell proliferation in differentiated pericyte-like cells, even under conditions of anoxic stress. Addition of the EGF receptor inhibitor AG 1478 led to complete inhibition of the proliferative effects of HB-EGF on pericyte-like cells. In addition, HB-EGF protected pericyte-like cells from anoxia-induced apoptosis. In addition, HB-EGF promoted cell proliferation in primary pericyte cultures. In vivo, administration of HB-EGF to mice subjected to intestinal I/R injury led to protection of pericytes from injury.
CONCLUSIONS: These results suggest that HB-EGF may function as a microcirculatory blood flow regulator, at least in part, via its effects on pericytes.
Authors:
Xiaoyi Yu; Andrei Radulescu; Chun-Liang Chen; Iyore O James; Gail E Besner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-17
Journal Detail:
Title:  The Journal of surgical research     Volume:  172     ISSN:  1095-8673     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-14     Completed Date:  2012-02-14     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-76     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / physiopathology
Apoptosis / drug effects*,  physiology
Cell Differentiation / drug effects
Cell Line
Cell Proliferation / drug effects*
Cells, Cultured
Intercellular Signaling Peptides and Proteins / pharmacology*
Intestines / blood supply
Male
Mesoderm / cytology,  drug effects
Mice
Mice, Inbred C3H
Models, Animal
Pericytes / cytology*,  drug effects*,  physiology
Rats
Rats, Sprague-Dawley
Reperfusion Injury / pathology,  physiopathology,  prevention & control
Transforming Growth Factor beta1 / pharmacology
Grant Support
ID/Acronym/Agency:
R01 DK074611/DK/NIDDK NIH HHS; R01 DK074611-05/DK/NIDDK NIH HHS; R01 GM061193/GM/NIGMS NIH HHS; R01 GM061193-08/GM/NIGMS NIH HHS; R01DK074611/DK/NIDDK NIH HHS; R01GM61193/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Transforming Growth Factor beta1; 149176-25-0/heparin-binding EGF-like growth factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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