| Heparin-binding EGF-like growth factor increases intestinal microvascular blood flow in necrotizing enterocolitis. | |
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MedLine Citation:
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PMID: 19361505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in neonates. Although the exact etiology remains unknown, decreased intestinal blood flow is believed to play a critical role. We have shown that heparin-binding epidermal growth factor-like growth factor (HB-EGF) protects the intestines from injury in a rodent model of NEC. Our current goal was to assess the effect of HB-EGF on intestinal microvascular blood flow and intestinal injury in rat pups subjected to experimental NEC. METHODS: Newborn rat pups were subjected to stress by exposure to hypoxia, hypothermia, hypertonic feedings, and lipopolysaccharide, with some pups receiving HB-EGF (800 microg x kg(-1) x dose(-1)) added to the feeds. Control animals received breast milk. Intestinal injury was graded using a standard histologic injury scoring system. Microvascular blood flow was assessed by fluorescein isothiocyanate/dextran angiography, with fluorescent images subjected to quantification, and by scanning electron microscopy. RESULTS: Intestinal microvascular blood flow (defined as the extent of vascular filling with fluorescein isothiocyanate/dextran) was significantly decreased in pups subjected to stress compared with breast-fed pups. Stressed pups treated with HB-EGF had significantly increased microvascular blood flow. The changes in villous microvasculature correlated with histologic injury scores, with stressed pups treated with HB-EGF showing decreased histologic injury. CONCLUSIONS: HB-EGF significantly preserved intestinal microvascular blood flow in pups subjected to experimental NEC, indicating that HB-EGF may play a critical role in the treatment of various diseases manifested by decreased intestinal blood flow, including NEC. |
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Authors:
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Xiaoyi Yu; Andrei Radulescu; Nicholas Zorko; Gail E Besner |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-04-08 |
Journal Detail:
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Title: Gastroenterology Volume: 137 ISSN: 1528-0012 ISO Abbreviation: Gastroenterology Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-30 Completed Date: 2009-07-16 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 221-30 Citation Subset: AIM; IM |
Affiliation:
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Department of Pediatric Surgery, Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, and Ohio State University College of Medicine, Columbus, Ohio 43205, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Arterioles / metabolism, physiopathology Capillaries / metabolism, physiopathology Disease Models, Animal Enterocolitis, Necrotizing / metabolism*, pathology, physiopathology, prevention & control Fluorescein Angiography Intercellular Signaling Peptides and Proteins / metabolism* Intestinal Mucosa / blood supply*, ultrastructure Intestines / blood supply*, ultrastructure Microcirculation* Microscopy, Confocal Microscopy, Electron, Scanning Microscopy, Fluorescence Microvilli / metabolism Rats Rats, Sprague-Dawley Severity of Illness Index Splanchnic Circulation* Venules / metabolism, physiopathology |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK074611/DK/NIDDK NIH HHS; R01 DK074611-01A2/DK/NIDDK NIH HHS; R01 DK074611-02/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Intercellular Signaling Peptides and Proteins; 149176-25-0/heparin-binding EGF-like growth factor |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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