Document Detail

Heparanase 1: a key participant of inner root sheath differentiation program and hair follicle homeostasis.
MedLine Citation:
PMID:  18557927     Owner:  NLM     Status:  MEDLINE    
Heparanase is a heparan sulphate endo-glycosidase which was previously detected in the outer root sheath of murine hair follicles. Heparanase overexpression was reported to improve mouse hair (re)growth. In this study, we investigated its involvement in human hair biology. Immunofluorescence detection was used to explore heparanase distribution in both anagen and catagen hair follicles. Heparanase functionality was assessed in in vitro cultured hair follicles, in the presence of a heparanase activity inhibitor. Our results showed that heparanase expression was (i) primarily located in the inner root sheath (IRS) of human hair follicle, and there (ii) restricted to anagen phase. Furthermore, inhibition of heparanase in in vitro cultured hair follicles induced a catagen-like process. Hair shaft retreat upward was accompanied by a decrease in Ki67-positive cells, the formation of an epithelial strand as evidenced by K14 keratin expression, and the loss of IRS as assessed by transglutaminase 1 and desmoglein labelling. IRS distribution of heparanase and the induction of catagen-like involution of hair follicles when a potent heparanase inhibitor is added suggest that heparanase is a key actor of IRS differentiation and hair homeostasis.
Sylvain Malgouries; Mark Donovan; Sébastien Thibaut; Bruno A Bernard
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2008-06-14
Journal Detail:
Title:  Experimental dermatology     Volume:  17     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2009-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  1017-23     Citation Subset:  IM    
L'Oréal Recherche, Clichy, France.
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MeSH Terms
Cell Differentiation / physiology*
Desmoglein 1 / metabolism
Enzyme Inhibitors / pharmacology
Epidermis / metabolism
Epithelial Cells / cytology,  drug effects,  metabolism
Fibronectins / metabolism
Hair Follicle / cytology,  drug effects,  physiology*
Heparin Lyase / antagonists & inhibitors,  metabolism*
Heparitin Sulfate / metabolism
Homeostasis / physiology*
Keratin-14 / metabolism
Keratins, Hair-Specific / metabolism
Keratins, Type I / metabolism
Ki-67 Antigen / metabolism
Middle Aged
Tissue Culture Techniques
Transglutaminases / metabolism
Reg. No./Substance:
0/DSG1 protein, human; 0/Desmoglein 1; 0/Enzyme Inhibitors; 0/Fibronectins; 0/KRT35 protein, human; 0/Keratin-14; 0/Keratins, Hair-Specific; 0/Keratins, Type I; 0/Ki-67 Antigen; 9050-30-0/Heparitin Sulfate; EC; EC 1; EC Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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