Document Detail


The HepaRG cell line: biological properties and relevance as a tool for cell biology, drug metabolism, and virology studies.
MedLine Citation:
PMID:  20645056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liver progenitor cells may play an important role in carcinogenesis in vivo and represent therefore useful cellular materials for in vitro studies. The HepaRG cell line, which is a human bipotent progenitor cell line capable to differentiate toward two different cell phenotypes (i.e., biliary-like and hepatocyte-like cells), has been established from a liver tumor associated with chronic hepatitis C. This cell line represents a valuable alternative to ex vivo cultivated primary human hepatocytes (PHH), as HepaRG cells share some features and properties with adult hepatocytes. The cell line is particularly useful to evaluate drugs and perform drug metabolism studies, as many detoxifying enzymes are expressed and functional. It is also an interesting tool to study some aspect of progenitor biology (e.g., differentiation process), carcinogenesis, and the infection by some pathogens for which the cell line is permissive (e.g., HBV infection). Overall, this chapter gives a concise overview of the biological properties and potential applications of this cell line.
Authors:
Marie-Jeanne Marion; Olivier Hantz; David Durantel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  640     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  261-72     Citation Subset:  IM    
Affiliation:
INSERM U871, Molecular physiopathology and new treatments of viral hepatitis, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Cell Line, Tumor* / cytology,  metabolism,  virology
Genotype
Hepacivirus / physiology
Hepatitis B / virology
Hepatitis B virus / physiology
Hepatitis C / virology
Hepatocytes* / cytology,  metabolism,  virology
Host-Pathogen Interactions
Humans
Liver / cytology,  metabolism,  virology
Liver Neoplasms* / virology
Pharmaceutical Preparations / metabolism
Phenotype
Protein Biosynthesis
Stem Cells* / cytology,  metabolism,  virology
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Pharmaceutical Preparations

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