| The HepaRG cell line: biological properties and relevance as a tool for cell biology, drug metabolism, and virology studies. | |
| | |
MedLine Citation:
|
PMID: 20645056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Liver progenitor cells may play an important role in carcinogenesis in vivo and represent therefore useful cellular materials for in vitro studies. The HepaRG cell line, which is a human bipotent progenitor cell line capable to differentiate toward two different cell phenotypes (i.e., biliary-like and hepatocyte-like cells), has been established from a liver tumor associated with chronic hepatitis C. This cell line represents a valuable alternative to ex vivo cultivated primary human hepatocytes (PHH), as HepaRG cells share some features and properties with adult hepatocytes. The cell line is particularly useful to evaluate drugs and perform drug metabolism studies, as many detoxifying enzymes are expressed and functional. It is also an interesting tool to study some aspect of progenitor biology (e.g., differentiation process), carcinogenesis, and the infection by some pathogens for which the cell line is permissive (e.g., HBV infection). Overall, this chapter gives a concise overview of the biological properties and potential applications of this cell line. |
| | |
Authors:
|
Marie-Jeanne Marion; Olivier Hantz; David Durantel |
Related Documents
:
|
3485686 - Purification to homogeneity and nh2-terminal amino acid sequence of a novel interleukin... 21152526 - Combination of fluid and solid mechanical stresses contribute to cell death and detachm... 16989856 - New cell lines from lymantria xylina (lepidoptera: lymantriidae): characterization and ... 52426 - Antigenic determinants common to established human b-cell lines, but not shared by huma... 2520196 - Effects of asymmetric division on a stochastic model of the cell division cycle. 23560766 - Dynamic evaluation of cell surface n-glycan expression via electrogenerated chemilumine... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 640 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-07-20 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 261-72 Citation Subset: IM |
Affiliation:
|
INSERM U871, Molecular physiopathology and new treatments of viral hepatitis, Lyon, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Differentiation Cell Line, Tumor* / cytology, metabolism, virology Genotype Hepacivirus / physiology Hepatitis B / virology Hepatitis B virus / physiology Hepatitis C / virology Hepatocytes* / cytology, metabolism, virology Host-Pathogen Interactions Humans Liver / cytology, metabolism, virology Liver Neoplasms* / virology Pharmaceutical Preparations / metabolism Phenotype Protein Biosynthesis Stem Cells* / cytology, metabolism, virology Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
|
0/Pharmaceutical Preparations |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and culture of adult human liver progenitor cells: in vitro differentiation to hepatocyte-...
Next Document: Transdifferentiation of pancreatic cells to hepatocytes.