| Hep3B human hepatoma cells support replication of the wild-type and a 5'-end deletion mutant GB virus B replicon. | |
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MedLine Citation:
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PMID: 14581524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hepatitis C virus (HCV) and GB virus B (GBV-B) replicons have been reported to replicate only in Huh7 cells. Here we demonstrate that subpopulations of another human hepatoma cell line, Hep3B, are permissive for the GBV-B replicon, showing different levels of enhancement of replication from those of the unselected parental cell population. Adaptive mutations are not required for replication of the GBV-B replicon in these cells, as already demonstrated for Huh7 cells. Nonetheless, we identified a mutant replicon in one of the selected cell lines, which, although lacking the 5' end proximal stem-loop, is able to replicate in Hep3B cells as well as in Huh7 cells. This mutant indeed shows a higher replication efficiency than does wild-type replicon, especially in the Hep3B cell clone from which it was originally recovered. This indicates that the stem-loop Ia is not necessary for replication of the GBV-B replicon in human cells, unlike what occurs with HCV, and that its absence can even provide a selective advantage. |
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Authors:
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Amedeo De Tomassi; Maura Pizzuti; Cinzia Traboni |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of virology Volume: 77 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-10-28 Completed Date: 2003-11-25 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 11875-81 Citation Subset: IM |
Affiliation:
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Istituto di Ricerche di Biologia Molecolare P. Angeletti, 00040 Pomezia (Rome), Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5' Untranslated Regions
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chemistry Carcinoma, Hepatocellular / virology* Cell Line, Tumor GB virus B / genetics, physiology* Humans Liver Neoplasms / virology* Mutation Protein Biosynthesis RNA, Viral / chemistry Replicon* Virus Replication* |
| Chemical | |
Reg. No./Substance:
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0/5' Untranslated Regions; 0/RNA, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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