Document Detail


Hemopoietic and angiogenetic progenitors in healthy athletes: different responses to endurance and maximal exercise.
MedLine Citation:
PMID:  20448032     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of endurance or maximal exercise on mobilization of bone marrow-derived hemopoietic and angiogenetic progenitors in healthy subjects are poorly defined. In 10 healthy amateur runners, we collected venous blood before, at the end of, and the day after a marathon race (n = 9), and before and at the end of a 1.5-km field test (n = 8), and measured hemopoietic and angiogenetic progenitors by flow cytometry and culture assays, as well as plasma or serum concentrations of several cytokines/growth factors. After the marathon, CD34(+) cells were unchanged, whereas clonogenetic assays showed decreased number of colonies for both erythropoietic (BFU-E) and granulocyte-monocyte (CFU-GM) series, returning to baseline the morning post-race. Conversely, CD34(+) cells, BFU-E, and CFU-GM increased after the field test. Angiogenetic progenitors, assessed as CD34(+)KDR(+) and CD133(+)VE-cadherin(+) cells or as adherent cells in culture expressing endothelial markers, increased after both endurance and maximal exercise but showed a different pattern between protocols. Interleukin-6 increased more after the marathon than after the field test, whereas hepatocyte growth factor and stem cell factor increased similarly in both protocols. Plasma levels of angiopoietin (Ang) 1 and 2 increased after both types of exercise, whereas the Ang-1-to-Ang-2 ratio or vascular endothelial growth factor-A were little affected. These data suggest that circulating hemopoietic progenitors may be utilized in peripheral tissues during prolonged endurance exercise. Endothelial progenitor mobilization after exercise in healthy trained subjects appears modulated by the type of exercise. Exercise-induced increase in growth factors suggests a physiological trophic effect of exercise on the bone marrow.
Authors:
Maria R Bonsignore; Giuseppe Morici; Roberta Riccioni; Alice Huertas; Eleonora Petrucci; Mario Veca; Gualtiero Mariani; Anna Bonanno; Laura Chimenti; Maria Gioia; Paolo Palange; Ugo Testa
Publication Detail:
Type:  Journal Article     Date:  2010-05-06
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  109     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-30     Completed Date:  2010-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  60-7     Citation Subset:  IM    
Affiliation:
Biomedical Department, Internal and Specialistic Medicine (DIBIMIS), Section of Pneumology, University of Palermo, Via Trabucco, 180, 90146 Palermo, Italy. marisa@ibim.cnr.it
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MeSH Terms
Descriptor/Qualifier:
Adult
Angiogenesis Inducing Agents / blood
Antigens, CD / blood
Antigens, CD34 / blood
Athletes*
Cadherins / blood
Cytokines / blood
Endothelial Cells / physiology*
Erythroid Precursor Cells / physiology*
Glycoproteins / blood
Granulocytes / physiology
Hematopoietic Cell Growth Factors / blood
Hematopoietic Stem Cells / physiology*
Humans
Male
Middle Aged
Neovascularization, Physiologic*
Peptides / blood
Physical Endurance / physiology*
Running / physiology
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Angiogenesis Inducing Agents; 0/Antigens, CD; 0/Antigens, CD34; 0/Cadherins; 0/Cytokines; 0/Glycoproteins; 0/Hematopoietic Cell Growth Factors; 0/Peptides

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