Document Detail


Hemolytic activity is mediated by the endogenous lectin in the mosquito hemolymph serum.
MedLine Citation:
PMID:  20193690     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Although cytolysis of invading organisms is an innate form of immunity used by invertebrates, so far the underlying mechanism remains less explored. The pupal hemolymph of the mosquito Armigeres subalbatus induces an activity that causes hemolysis of human red blood cells (HRBC). This hemolytic activity was inhibited by sialic acid (N-acetylneuraminic acid) and serine protease inhibitors. We purified the sialic acid-specific lectin(s) from the pupal hemolymph using formaldehyde-fixed HRBC and determined the sequence of the amino-terminal 19 amino acid residues. A polyclonal antibody produced against this N-terminal peptide clearly inhibited the hemolytic activity of the hemolymph in vitro, thus suggesting that the hemolysis of HRBC is caused by the lectin present in the mosquito hemolymph. We suggest that mosquitoes possess a cytolysis system.
Authors:
Toshinori Sasaki; Tsuyoshi Hiraoka; Mutsuo Kobayashi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-07
Journal Detail:
Title:  Journal of insect physiology     Volume:  56     ISSN:  1879-1611     ISO Abbreviation:  J. Insect Physiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985080R     Medline TA:  J Insect Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1032-9     Citation Subset:  IM    
Affiliation:
Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. tsasaki@nih.go.jp
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