| Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production. | |
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MedLine Citation:
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PMID: 20023108 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pseudomonas entomophila is an entomopathogenic bacterium that is able to infect and kill Drosophila melanogaster upon ingestion. Its genome sequence suggests that it is a versatile soil bacterium closely related to Pseudomonas putida. The GacS/GacA two-component system plays a key role in P. entomophila pathogenicity, controlling many putative virulence factors and AprA, a secreted protease important to escape the fly immune response. P. entomophila secretes a strong diffusible hemolytic activity. Here, we showed that this activity is linked to the production of a new cyclic lipopeptide containing 14 amino acids and a 3-C(10)OH fatty acid that we called entolysin. Three nonribosomal peptide synthetases (EtlA, EtlB, EtlC) were identified as responsible for entolysin biosynthesis. Two additional components (EtlR, MacAB) are necessary for its production and secretion. The P. entomophila GacS/GacA two-component system regulates entolysin production, and we demonstrated that its functioning requires two small RNAs and two RsmA-like proteins. Finally, entolysin is required for swarming motility, as described for other lipopeptides, but it does not participate in the virulence of P. entomophila for Drosophila. While investigating the physiological role of entolysin, we also uncovered new phenotypes associated with P. entomophila, including strong biocontrol abilities. |
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Authors:
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Isabelle Vallet-Gely; Alexey Novikov; Luis Augusto; Peter Liehl; Gérard Bolbach; Maria Péchy-Tarr; Pierre Cosson; Christoph Keel; Martine Caroff; Bruno Lemaitre |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-18 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-28 Completed Date: 2010-04-16 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 910-21 Citation Subset: IM |
Affiliation:
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Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette, France. vallet@cgm.cnrs-gif.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Bacterial Proteins / genetics, metabolism Drosophila / genetics, metabolism Endopeptidase Clp / genetics, metabolism Endopeptidases / genetics, metabolism Gene Expression Regulation, Bacterial Genes, Bacterial Genome, Bacterial Hemolytic Agents / chemistry, metabolism* Lipopeptides / biosynthesis*, chemistry, metabolism Peptides, Cyclic / biosynthesis*, chemistry, metabolism Pest Control, Biological Pseudomonas / genetics*, metabolism, pathogenicity RNA, Bacterial / genetics, metabolism RNA, Untranslated / genetics, metabolism Repressor Proteins / genetics, metabolism Soil Microbiology* Virulence / genetics Virulence Factors / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Hemolytic Agents; 0/Lipopeptides; 0/Peptides, Cyclic; 0/RNA, Bacterial; 0/RNA, Untranslated; 0/Repressor Proteins; 0/Virulence Factors; EC 3.4.-/Endopeptidases; EC 3.4.21.92/Endopeptidase Clp |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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