Document Detail


Association of hemolytic activity of Pseudomonas entomophila, a versatile soil bacterium, with cyclic lipopeptide production.
MedLine Citation:
PMID:  20023108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas entomophila is an entomopathogenic bacterium that is able to infect and kill Drosophila melanogaster upon ingestion. Its genome sequence suggests that it is a versatile soil bacterium closely related to Pseudomonas putida. The GacS/GacA two-component system plays a key role in P. entomophila pathogenicity, controlling many putative virulence factors and AprA, a secreted protease important to escape the fly immune response. P. entomophila secretes a strong diffusible hemolytic activity. Here, we showed that this activity is linked to the production of a new cyclic lipopeptide containing 14 amino acids and a 3-C(10)OH fatty acid that we called entolysin. Three nonribosomal peptide synthetases (EtlA, EtlB, EtlC) were identified as responsible for entolysin biosynthesis. Two additional components (EtlR, MacAB) are necessary for its production and secretion. The P. entomophila GacS/GacA two-component system regulates entolysin production, and we demonstrated that its functioning requires two small RNAs and two RsmA-like proteins. Finally, entolysin is required for swarming motility, as described for other lipopeptides, but it does not participate in the virulence of P. entomophila for Drosophila. While investigating the physiological role of entolysin, we also uncovered new phenotypes associated with P. entomophila, including strong biocontrol abilities.
Authors:
Isabelle Vallet-Gely; Alexey Novikov; Luis Augusto; Peter Liehl; Gérard Bolbach; Maria Péchy-Tarr; Pierre Cosson; Christoph Keel; Martine Caroff; Bruno Lemaitre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-18
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  76     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-28     Completed Date:  2010-04-16     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  910-21     Citation Subset:  IM    
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91198 Gif-sur-Yvette, France. vallet@cgm.cnrs-gif.fr
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Bacterial Proteins / genetics,  metabolism
Drosophila / genetics,  metabolism
Endopeptidase Clp / genetics,  metabolism
Endopeptidases / genetics,  metabolism
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genome, Bacterial
Hemolytic Agents / chemistry,  metabolism*
Lipopeptides / biosynthesis*,  chemistry,  metabolism
Peptides, Cyclic / biosynthesis*,  chemistry,  metabolism
Pest Control, Biological
Pseudomonas / genetics*,  metabolism,  pathogenicity
RNA, Bacterial / genetics,  metabolism
RNA, Untranslated / genetics,  metabolism
Repressor Proteins / genetics,  metabolism
Soil Microbiology*
Virulence / genetics
Virulence Factors / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Hemolytic Agents; 0/Lipopeptides; 0/Peptides, Cyclic; 0/RNA, Bacterial; 0/RNA, Untranslated; 0/Repressor Proteins; 0/Virulence Factors; EC 3.4.-/Endopeptidases; EC 3.4.21.92/Endopeptidase Clp
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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