| Hemolysin induces Toll-like receptor (TLR) independent apoptosis but multiple TLR associated parallel activation of macrophages. | |
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MedLine Citation:
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PMID: 21846723 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Vibrio cholerae hemolysin (HlyA) displays bipartite property while supervising macrophages (Mϕ). The pore-forming toxin causes profound apoptosis within 3 h of exposure and in parallel supports activation of the defying Mϕ. HlyA-induced apoptosis of Mϕ remains steady for 24 h, is Toll-like receptor (TLR) independent and driven by caspase-9 and caspase-7 thus involves mitochondrial or intrinsic pathway. Cell activation is carried forward by time dependent up-regulation of varying TLRs. The promiscuous TLR association of HlyA prompted investigation which revealed β-prism lectin domain of HlyA simulated TLR4 up-regulation by jacalin, a plant lectin homologue besides expressing CD86 and type I cytokines tumor necrosis factor-α (TNF-α) and interleukin (IL)-12. However, HlyA cytolytic protein domain up-regulated TLR2, which controlled CD40 for continuity of cell activation. Expression of tollip before TLR2 and TLR6 abrogated TLR4, CD40 and CD86. We show that the transient expression of tollip leading to curbing of activation associated capabilities is a plausible feed-back mechanism of Mϕ to deploy TLR2 and prolong activation involving CD40 to encounter HlyA cytolysin domain. |
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Authors:
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Deep Chandan Chakraborty; Gayatri Mukherjee; Pallavi Banerjee; Kalyan K Banerjee; Tapas Biswas |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-16 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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National Institute of Cholera and Enteric Diseases, India. |
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