Document Detail


Hemokinin-1 has Substance P-like function in U-251 MG astrocytoma cells: a pharmacological and functional study.
MedLine Citation:
PMID:  15913794     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Substance P (SP) triggers responses in astrocytoma cells, which are considered important for proliferation and neuroimmunomodulatory activity. In this study, we compared the effects of SP with those of the novel tachykinin Hemokinin-1 (HK-1) in the human astrocytoma cell line U-251 MG. We show that U-251 MG cells express high levels of Neurokinin-1 (NK-1) receptors. The binding affinities of 125I-SP and 125I-mHK-1 to these receptors were in a similar, subnanomolar range. HK-1 and SP stimulated Ca2+ mobilization and induced increased cytokine mRNA expression. A specific NK-1 receptor antagonist blocked the observed effects. We conclude that there are no qualitative differences in SP and HK-1-evoked responses, suggesting that both peptides act through NK-1 receptors in U-251 MG cells. Moreover, we show TAC4 mRNA expression in gliomas, indicating a possible involvement of HK-1 in glioma biology.
Authors:
Alexandra Berger; Christopher J Paige
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  164     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-20     Completed Date:  2005-08-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  48-56     Citation Subset:  IM    
Affiliation:
Princess Margaret Hospital, Ontario Cancer Institute, University Health Network, Department of Immunology, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G 2M9. aberger@uhnres.utoronto.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytoma
Blotting, Northern / methods
Calcium / metabolism
Cell Count / methods
Cell Line, Tumor
Cytokines / genetics,  metabolism
Dose-Response Relationship, Drug
Gene Expression Regulation, Neoplastic / drug effects*
Hexokinase / pharmacokinetics,  pharmacology*
Humans
Immunohistochemistry / methods
Iodine Isotopes / pharmacokinetics
Mice
Protein Binding / drug effects
Protein Precursors / pharmacokinetics,  pharmacology*
RNA, Messenger / biosynthesis
Radioligand Assay / methods
Receptors, Neurokinin-1 / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods
Substance P / pharmacokinetics,  pharmacology*
Tachykinins / pharmacokinetics,  pharmacology*
Time Factors
Chemical
Reg. No./Substance:
0/Cytokines; 0/Iodine Isotopes; 0/Protein Precursors; 0/RNA, Messenger; 0/Receptors, Neurokinin-1; 0/Tac4 protein, mouse; 0/Tachykinins; 33507-63-0/Substance P; 7440-70-2/Calcium; EC 2.7.1.1/HK1 protein, human; EC 2.7.1.1/Hexokinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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