Document Detail


Hemoglobin infusion does not alter murine pulmonary vascular tone.
MedLine Citation:
PMID:  23313572     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasma hemoglobin (Hb) scavenges endothelium-derived nitric oxide (NO), producing systemic and pulmonary vasoconstriction in many species. We hypothesized that i.v. administration of murine cell-free Hb would produce pulmonary vasoconstriction and enhance hypoxic pulmonary vasoconstriction (HPV) in mice. To assess the impact of plasma Hb on basal pulmonary vascular tone in anesthetized mice we measured left lung pulmonary vascular resistance (LPVRI) before and after infusion of Hb at thoracotomy. To confirm the findings obtained at thoracotomy, measurements of right ventricular systolic pressure (RVSP) and systemic arterial pressure (SAP) were obtained in closed-chest wild-type mice. To elucidate whether pretreatment with Hb augments HPV we assessed the increase in LPVRI before and during regional lung hypoxia produced by left mainstem bronchial occlusion (LMBO) in wild-type mice pretreated with Hb. Infusion of Hb increased SAP but did not change pulmonary arterial pressure (PAP), left lung pulmonary arterial flow (QLPA) or LPVRI in either wild-type or diabetic mice with endothelial dysfunction. Scavenging of NO by plasma Hb did not alter HPV in wild-type mice. Inhibition of NO synthase with l-NAME did not change the basal LPVRI, but augmented HPV during LMBO. Our data suggest that scavenging of NO by plasma Hb does not alter pulmonary vascular tone in mice. Therefore, generation of NO in the pulmonary circulation is unlikely to be responsible for the low basal pulmonary vascular tone of mice.
Authors:
Arkadi Beloiartsev; David M Baron; Binglan Yu; Kenneth D Bloch; Warren M Zapol
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-08
Journal Detail:
Title:  Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society     Volume:  30     ISSN:  1089-8611     ISO Abbreviation:  Nitric Oxide     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-03     Completed Date:  2013-09-23     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  9709307     Medline TA:  Nitric Oxide     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
Analysis of Variance
Animals
Anoxia
Blood Pressure / drug effects
Diabetes Mellitus, Experimental
Hemoglobins / administration & dosage*
Lung / blood supply*,  chemistry,  drug effects*,  metabolism
Male
Mice
Mice, Inbred C57BL
NG-Nitroarginine Methyl Ester / metabolism
Nitric Oxide / metabolism
Nitric Oxide Synthase / metabolism
Superoxides / metabolism
Thoracotomy
Vascular Resistance / drug effects*
Vasoconstriction / drug effects*
Vasoconstrictor Agents / pharmacology
Grant Support
ID/Acronym/Agency:
HL074352/HL/NHLBI NIH HHS; R01 HL074352/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobins; 0/Vasoconstrictor Agents; 11062-77-4/Superoxides; 31C4KY9ESH/Nitric Oxide; 76898-47-0/15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; EC 1.14.13.39/Nitric Oxide Synthase; V55S2QJN2X/NG-Nitroarginine Methyl Ester
Comments/Corrections

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