Document Detail


Hemoglobin induction by Ara-C in human erythroleukemic cells (K562) is cell-cycle dependent.
MedLine Citation:
PMID:  2796376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was undertaken to answer the question of whether chemical induction of hemoglobin in the human erythroleukemic line K562 is cell-cycle dependent. Cells were synchronized with respect to the cell cycle by manipulation of culture conditions. S phase was detected microscopically by immunofluorescent staining. Hemin and arabinofuranosylcytosine (Ara-C) were used to induce hemoglobin synthesis in K562 cells. The action of hemin causes induction without specificity in regard to cell cycle phase, while Ara-C is dependent upon cell cycle phase, with cells within late G1 and early S phases being sensitive to the effect of the agent. These studies also confirm the results that the action of hemin is reversible but the induction caused by Ara-C is irreversible. The synergistic effects of these two inducing agents resulted in a sharper rise in the percentage of induced cells.
Authors:
P E Wanda; M M Walker
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Leukemia research     Volume:  13     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1989  
Date Detail:
Created Date:  1989-11-01     Completed Date:  1989-11-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  683-8     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Southern Illinois University, Edwardsville 62026-1651.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / drug effects*
Cell Line
Cytarabine / pharmacology*
Heme / pharmacology
Hemoglobins / biosynthesis*
Humans
Hydroxyurea / pharmacology
Interphase / drug effects
Kinetics
Leukemia, Erythroblastic, Acute / metabolism*,  pathology
Grant Support
ID/Acronym/Agency:
R15-A123103-01//PHS HHS
Chemical
Reg. No./Substance:
0/Hemoglobins; 127-07-1/Hydroxyurea; 147-94-4/Cytarabine; 14875-96-8/Heme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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