Document Detail

Hemoglobin induction by Ara-C in human erythroleukemic cells (K562) is cell-cycle dependent.
MedLine Citation:
PMID:  2796376     Owner:  NLM     Status:  MEDLINE    
This study was undertaken to answer the question of whether chemical induction of hemoglobin in the human erythroleukemic line K562 is cell-cycle dependent. Cells were synchronized with respect to the cell cycle by manipulation of culture conditions. S phase was detected microscopically by immunofluorescent staining. Hemin and arabinofuranosylcytosine (Ara-C) were used to induce hemoglobin synthesis in K562 cells. The action of hemin causes induction without specificity in regard to cell cycle phase, while Ara-C is dependent upon cell cycle phase, with cells within late G1 and early S phases being sensitive to the effect of the agent. These studies also confirm the results that the action of hemin is reversible but the induction caused by Ara-C is irreversible. The synergistic effects of these two inducing agents resulted in a sharper rise in the percentage of induced cells.
P E Wanda; M M Walker
Related Documents :
8218356 - Effects of osmotic stress and growth stage on cellular ph and polyphosphate metabolism ...
17271966 - Fractal analysis of replication site images of the human cell nucleus.
7042716 - Poly(adp-ribosyl)ation of chromosomal proteins in the hela s3 cell cycle.
12077326 - The sin3/rpd3 deacetylase complex is essential for g(2) phase cell cycle progression an...
12546876 - Applications of transfected cell microarrays in high-throughput drug discovery.
7169496 - Butyrate-induced histone hyperacetylation in human and mouse cells: estimation of putat...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Leukemia research     Volume:  13     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1989  
Date Detail:
Created Date:  1989-11-01     Completed Date:  1989-11-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  683-8     Citation Subset:  IM    
Department of Biological Sciences, Southern Illinois University, Edwardsville 62026-1651.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Cycle / drug effects*
Cell Line
Cytarabine / pharmacology*
Heme / pharmacology
Hemoglobins / biosynthesis*
Hydroxyurea / pharmacology
Interphase / drug effects
Leukemia, Erythroblastic, Acute / metabolism*,  pathology
Grant Support
R15-A123103-01//PHS HHS
Reg. No./Substance:
0/Hemoglobins; 127-07-1/Hydroxyurea; 147-94-4/Cytarabine; 14875-96-8/Heme

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The pattern of myeloid suppression and recovery after the addition of methotrexate to murine long-te...
Next Document:  Establishment and partial characterization of a radiation-induced canine monocytic leukemic cell lin...